Identification of anthocyanin pigments in strawberry (cv Camarosa) by LC using DAD and ESI-MS detection
New high performance liquid chromatography (HPLC) conditions were developed for the separation of strawberry anthocyanins that provided a good resolution of peaks at a low flow rate compatible with the requirements of the mass spectrometry (MS) detector. A strawberry extract was fractionated by column chromatography and simple fractions containing basically anthocyanins were obtained, making their analysis by HPLC possible using on-line photodiode array detection and MS. Information on the identity of the major and some secondary anthocyanins in strawberry was obtained from their retention characteristics, UV-visible spectra and mass spectra. The presence in strawberry of the previously reported cyanidin 3-glucoside, pelargonidin 3-glucoside, pelargonidin 3-rutinoside and pelargonidin 3-acetylglucoside was confirmed and cyanidin 3-rutinoside was identified in strawberry for the first time. Furthermore, cyanidin 3-malonyldiglucoside, pelargonidin 3-malylglucoside, a pelargonidin bioside and two possible pelargonidin 3-biosides acylated with acetic acid were also tentatively assigned.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Springer Nature
2002
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| Online Access: | http://hdl.handle.net/10261/242221 http://dx.doi.org/10.13039/501100014180 http://dx.doi.org/10.13039/501100000780 |
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