Direct method for regiospecific analysis of TAG using [alpha]-MAG

An analytical procedure was developed for regiodistribution analysis of TAG using [alpha]-MAG prepared by an ethyl magnesium bromide deacylation. In the present communication, the deacylation procedure is shown to lead to representative [alpha]-MAG, allowing the composition of the native TAG in the a-position to be determined directly. The composition in the [bêta]-position can then be estimated from the composition of the [alpha]-MAG and TAG according to the formula 3 x TAG - 2 x [alpha]-MAG. The estimates are superior to those obtained using the [alpha],[bêta]-DAG and Brockerhoff calculations as they come closer to the theoretical value and have smaller SID. The present procedure, first demonstrated on a synthetic TAG, was then successfully applied to the analysis of borage oil, milkfat, and tuna oil.

Bibliographic Details

Main Authors:	Turon, Fabrice, Bachain, Philippe, Caro, Yanis, Pina, Michel, Graille, Jean
Format:	article biblioteca
Language:	eng
Subjects:	Q04 - Composition des produits alimentaires, U30 - Méthodes de recherche, composition chimique, corps gras, acylation, technique analytique, triglycéride, http://aims.fao.org/aos/agrovoc/c_1794, http://aims.fao.org/aos/agrovoc/c_2816, http://aims.fao.org/aos/agrovoc/c_28220, http://aims.fao.org/aos/agrovoc/c_1513, http://aims.fao.org/aos/agrovoc/c_14992,
Online Access:	http://agritrop.cirad.fr/515364/ http://agritrop.cirad.fr/515364/1/515364.pdf
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