Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage
To extend fruit market life, tomatoes are harvested before red ripe and kept at temperatures below optimum (20°C). In this work, Micro-Tom tomatoes stored at 20°C (normal ripening) were compared with those stored at 15°C or 4°C (chilling injury inducer) for 7 days. In contrast to 4°C, storage at 15°C delayed ripening with the benefit of not enhancing oxidative metabolism and of enabling ripening upon being transferred to 20°C. The transcriptional expression profile of enzymes related to cell wall metabolism was compared at the three temperatures. Although endo-β-1,4-glucanase (Cel1), which is associated with fruit decay, was largely increased after removal from 4°C storage, its expression was not modified in fruits stored at 15°C. Enhanced transcriptional expression of xyloglucan endotransgylcosylase/hydrolases (XTHs) XTH1, –2, –10 and –11, and of two β-xylosidases (Xyl1–2) was detected in fruits stored at 15°C with respect to those at 20°C. Following 2 days at 20°C, these transcripts remained higher in fruits stored at 15°C and XHT3 and –9 also increased. Ethylene evolution was similar in fruits kept at 15°C and 20°C; thus, the changes in the transcript profile and fruit properties between these treatments may be under the control of factors other than ethylene.
| Main Authors: | , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | eng |
| Published: |
2013
|
| Subjects: | Tomate, Solanum Lycopersicum, Fisiología Postcosecha, Maduración en Postcosecha, Pared Celular, Metabolismo, Almacenamiento en Frío, Aptitud para la Conservación, Tomatoes, Postharvest Physiology, Postharvest Ripening, Cell Walls, Metabolism, Cold Storage, Keeping Quality, Metabolismo de la Pared Celular, Daño por Frío, Endotransglicosilasa/Hidrolasa, Xyloglucan β-xilosidasa, Cell Wall Metabolism, Chilling Injury, Endotransgylcosylase/Hydrolase, Xyloglucan β-xylosidase, |
| Online Access: | http://www.publish.csiro.au/FP/FP12272 http://hdl.handle.net/20.500.12123/3213 https://doi.org/10.1071/FP12272 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|