Phenotypic and genetic evaluations of biogenic amine production by lactic acid bacteria isolated from fish and fish products

In this work, biogenic amine production (histamine, tyramine and putrescine) by a collection of 74 lactic acid bacteria of aquatic origin has been investigated by means of amino acid decarboxylation by growth on decarboxylase differential medium, biogenic amine detection by thin-layer chromatography (TLC) and decarboxylase gene detection by PCR. None of the evaluated strains showed neither production of histamine and putrescine, nor presence of the genetic determinants encoding the corresponding decarboxylase activities. However, the tyrosine decarboxylase gene (tdc) was present in all the enterococcal strains, and tyramine production was detected by TLC in all of them but Enterococcus faecium BCS59 and MV5. Analysis of the tyrosine decarboxylase operon of these strains revealed the presence of an insertion sequence upstream tdc that could be responsible for their lack of tyrosine decarboxylase activity. © 2011 Elsevier B.V.

Bibliographic Details

Main Authors:	Muñoz-Atienza, E., Landeta, Gerardo, Rivas, Blanca de las, Gómez-Sala, Beatriz, Muñoz, Rosario, Hernández, Pablo E., Cintas, Luis M., Herranz, Carmen
Other Authors:	Ministerio de Ciencia y Tecnología (España)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2011-03-30
Online Access:	http://hdl.handle.net/10261/60282 http://dx.doi.org/10.13039/501100006280 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003141 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/100012818
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