Protective effect of bioaccessible fractions of citrus fruit pulps against H2O2-induced oxidative stress in Caco-2 cells

Fruit pulps from Navel (N) and Cara Cara (CC) oranges, and Clementine mandarin freshly harvested (M) and refrigerated stored (M12) were used to evaluate the cytoprotective effect of their bioaccessible fractions (BF) against H2O2-induced oxidative stress in Caco-2 cells. BF of samples preserved viability vs. H2O2 treated cells, reaching values similar to controls. Lipid peroxidation was reduced to levels of control cells, but M did not reach control values. ROS and mitochondrial membrane potential changes (Δψm) values were reduced compared with H2O2 treated cells, but without achieving control levels. A significant reduction in cell proportions in G1 phase and a significant increase in sub-G1 phase (apoptosis) of cell cycle was shown in H2O2 treated cells, and BF allowed a recovery close to control levels. Thus, BF of samples protect the cells from oxidative stress by preserving cell viability, mitochondrial membrane potential and correct cell cycle progression, and diminishing lipid peroxidation and ROS.

Bibliographic Details

Main Authors:	Cilla, Antonio, Rodrigo, María Jesús, Zacarías, Lorenzo, Ancos, Begoña de, Sánchez-Moreno, Concepción, Barberá, Reyes, Alegría, Amparo
Other Authors:	Ministerio de Economía y Competitividad (España)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2017-10-31
Subjects:	Citrus fruit pulps, Bioactive compounds, Antioxidants, In vitro digestion, Caco-2 cells, Oxidative stress,
Online Access:	http://hdl.handle.net/10261/157604 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780
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