Analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (PAHs) in cocoa butter using HPLC-FLD
A simple, fast and ecological analytical method using a semi-automatic fat extractor and HPLC-FLD (fluorescence detection) for determination of polycyclic aromatic hydrocarbon markers i.e. benzo(a)anthracene (BaA), chrysene (Chr), benzo(a)pyrene (BaP) and benzo(b)fluoranthene (BbF) in cocoa butter has been validated. Validation's procedure performed out in concordance with French standard NF V03-110 (2010) was based on existing polycyclic aromatic hydrocarbon (PAH) determination methods in various smoked foodstuffs and edible vegetable oils. Determination of correlation coefficients for specific PAHs ranged from 0.9992 to 0.9998. Respective values of limits of detection were 0.010, 0.011, 0.033 and 0.029 μg kg−1 and those of quantification were 0.035, 0.038, 0.111 and 0.098 μg kg−1 for BaA, Chr, BbF and BaP. Both values of repeatability and intermediary precision tests coefficients of variation were less than 5%. Recovery scores of four PAH markers matched EU standard 836/2011 recommendations. Sum of four PAH markers (BaA, Chr, BbF, BaP) contents varied from 5.42 ± 0.58 to 11.37 ± 0.01 μg kg−1 whereas those of BaP was comprised between 0.26 ± 0.00 and 1.75 ± 0.13 μg kg−1 in 20 cocoa butter samples extracted from raw cocoa bean stored at Ivorian cocoa farmer levels.
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Subjects: | Q03 - Contamination et toxicologie alimentaires, U30 - Méthodes de recherche, P02 - Pollution, beurre de cacao, contamination chimique, hydrocarbure aromatique polycyclique, HPLC, fève de cacao, méthode par chimiluminescence, http://aims.fao.org/aos/agrovoc/c_28260, http://aims.fao.org/aos/agrovoc/c_28319, http://aims.fao.org/aos/agrovoc/c_331394, http://aims.fao.org/aos/agrovoc/c_24469, http://aims.fao.org/aos/agrovoc/c_1711, http://aims.fao.org/aos/agrovoc/c_35049, http://aims.fao.org/aos/agrovoc/c_4027, |
Online Access: | http://agritrop.cirad.fr/588508/ http://agritrop.cirad.fr/588508/7/n-durand-2018.pdf |
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dig-cirad-fr-5885082024-10-18T16:11:51Z http://agritrop.cirad.fr/588508/ http://agritrop.cirad.fr/588508/ Analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (PAHs) in cocoa butter using HPLC-FLD. Sess Tchotch Didier Axel, Kedjebo Kra Brou Didier, Faulet Betty Meuwiah, Fontana-Tachon Angélique, Alter Pascaline, Durand Noel, Grabulos Joël, Montet Didier, Guehi Tagro Simplice. 2018. Food Analytical Methods, 11 (11) : 3138-3146.https://doi.org/10.1007/s12161-018-1282-2 <https://doi.org/10.1007/s12161-018-1282-2> Analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (PAHs) in cocoa butter using HPLC-FLD Sess Tchotch, Didier Axel Kedjebo, Kra Brou Didier Faulet, Betty Meuwiah Fontana-Tachon, Angélique Alter, Pascaline Durand, Noel Grabulos, Joël Montet, Didier Guehi, Tagro Simplice eng 2018 Food Analytical Methods Q03 - Contamination et toxicologie alimentaires U30 - Méthodes de recherche P02 - Pollution beurre de cacao contamination chimique hydrocarbure aromatique polycyclique HPLC fève de cacao méthode par chimiluminescence http://aims.fao.org/aos/agrovoc/c_28260 http://aims.fao.org/aos/agrovoc/c_28319 http://aims.fao.org/aos/agrovoc/c_331394 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_1711 http://aims.fao.org/aos/agrovoc/c_35049 Côte d'Ivoire http://aims.fao.org/aos/agrovoc/c_4027 A simple, fast and ecological analytical method using a semi-automatic fat extractor and HPLC-FLD (fluorescence detection) for determination of polycyclic aromatic hydrocarbon markers i.e. benzo(a)anthracene (BaA), chrysene (Chr), benzo(a)pyrene (BaP) and benzo(b)fluoranthene (BbF) in cocoa butter has been validated. Validation's procedure performed out in concordance with French standard NF V03-110 (2010) was based on existing polycyclic aromatic hydrocarbon (PAH) determination methods in various smoked foodstuffs and edible vegetable oils. Determination of correlation coefficients for specific PAHs ranged from 0.9992 to 0.9998. Respective values of limits of detection were 0.010, 0.011, 0.033 and 0.029 μg kg−1 and those of quantification were 0.035, 0.038, 0.111 and 0.098 μg kg−1 for BaA, Chr, BbF and BaP. Both values of repeatability and intermediary precision tests coefficients of variation were less than 5%. Recovery scores of four PAH markers matched EU standard 836/2011 recommendations. Sum of four PAH markers (BaA, Chr, BbF, BaP) contents varied from 5.42 ± 0.58 to 11.37 ± 0.01 μg kg−1 whereas those of BaP was comprised between 0.26 ± 0.00 and 1.75 ± 0.13 μg kg−1 in 20 cocoa butter samples extracted from raw cocoa bean stored at Ivorian cocoa farmer levels. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/588508/7/n-durand-2018.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s12161-018-1282-2 10.1007/s12161-018-1282-2 info:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-018-1282-2 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s12161-018-1282-2 |
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Q03 - Contamination et toxicologie alimentaires U30 - Méthodes de recherche P02 - Pollution beurre de cacao contamination chimique hydrocarbure aromatique polycyclique HPLC fève de cacao méthode par chimiluminescence http://aims.fao.org/aos/agrovoc/c_28260 http://aims.fao.org/aos/agrovoc/c_28319 http://aims.fao.org/aos/agrovoc/c_331394 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_1711 http://aims.fao.org/aos/agrovoc/c_35049 http://aims.fao.org/aos/agrovoc/c_4027 Q03 - Contamination et toxicologie alimentaires U30 - Méthodes de recherche P02 - Pollution beurre de cacao contamination chimique hydrocarbure aromatique polycyclique HPLC fève de cacao méthode par chimiluminescence http://aims.fao.org/aos/agrovoc/c_28260 http://aims.fao.org/aos/agrovoc/c_28319 http://aims.fao.org/aos/agrovoc/c_331394 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_1711 http://aims.fao.org/aos/agrovoc/c_35049 http://aims.fao.org/aos/agrovoc/c_4027 |
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Q03 - Contamination et toxicologie alimentaires U30 - Méthodes de recherche P02 - Pollution beurre de cacao contamination chimique hydrocarbure aromatique polycyclique HPLC fève de cacao méthode par chimiluminescence http://aims.fao.org/aos/agrovoc/c_28260 http://aims.fao.org/aos/agrovoc/c_28319 http://aims.fao.org/aos/agrovoc/c_331394 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_1711 http://aims.fao.org/aos/agrovoc/c_35049 http://aims.fao.org/aos/agrovoc/c_4027 Q03 - Contamination et toxicologie alimentaires U30 - Méthodes de recherche P02 - Pollution beurre de cacao contamination chimique hydrocarbure aromatique polycyclique HPLC fève de cacao méthode par chimiluminescence http://aims.fao.org/aos/agrovoc/c_28260 http://aims.fao.org/aos/agrovoc/c_28319 http://aims.fao.org/aos/agrovoc/c_331394 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_1711 http://aims.fao.org/aos/agrovoc/c_35049 http://aims.fao.org/aos/agrovoc/c_4027 Sess Tchotch, Didier Axel Kedjebo, Kra Brou Didier Faulet, Betty Meuwiah Fontana-Tachon, Angélique Alter, Pascaline Durand, Noel Grabulos, Joël Montet, Didier Guehi, Tagro Simplice Analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (PAHs) in cocoa butter using HPLC-FLD |
description |
A simple, fast and ecological analytical method using a semi-automatic fat extractor and HPLC-FLD (fluorescence detection) for determination of polycyclic aromatic hydrocarbon markers i.e. benzo(a)anthracene (BaA), chrysene (Chr), benzo(a)pyrene (BaP) and benzo(b)fluoranthene (BbF) in cocoa butter has been validated. Validation's procedure performed out in concordance with French standard NF V03-110 (2010) was based on existing polycyclic aromatic hydrocarbon (PAH) determination methods in various smoked foodstuffs and edible vegetable oils. Determination of correlation coefficients for specific PAHs ranged from 0.9992 to 0.9998. Respective values of limits of detection were 0.010, 0.011, 0.033 and 0.029 μg kg−1 and those of quantification were 0.035, 0.038, 0.111 and 0.098 μg kg−1 for BaA, Chr, BbF and BaP. Both values of repeatability and intermediary precision tests coefficients of variation were less than 5%. Recovery scores of four PAH markers matched EU standard 836/2011 recommendations. Sum of four PAH markers (BaA, Chr, BbF, BaP) contents varied from 5.42 ± 0.58 to 11.37 ± 0.01 μg kg−1 whereas those of BaP was comprised between 0.26 ± 0.00 and 1.75 ± 0.13 μg kg−1 in 20 cocoa butter samples extracted from raw cocoa bean stored at Ivorian cocoa farmer levels. |
format |
article |
topic_facet |
Q03 - Contamination et toxicologie alimentaires U30 - Méthodes de recherche P02 - Pollution beurre de cacao contamination chimique hydrocarbure aromatique polycyclique HPLC fève de cacao méthode par chimiluminescence http://aims.fao.org/aos/agrovoc/c_28260 http://aims.fao.org/aos/agrovoc/c_28319 http://aims.fao.org/aos/agrovoc/c_331394 http://aims.fao.org/aos/agrovoc/c_24469 http://aims.fao.org/aos/agrovoc/c_1711 http://aims.fao.org/aos/agrovoc/c_35049 http://aims.fao.org/aos/agrovoc/c_4027 |
author |
Sess Tchotch, Didier Axel Kedjebo, Kra Brou Didier Faulet, Betty Meuwiah Fontana-Tachon, Angélique Alter, Pascaline Durand, Noel Grabulos, Joël Montet, Didier Guehi, Tagro Simplice |
author_facet |
Sess Tchotch, Didier Axel Kedjebo, Kra Brou Didier Faulet, Betty Meuwiah Fontana-Tachon, Angélique Alter, Pascaline Durand, Noel Grabulos, Joël Montet, Didier Guehi, Tagro Simplice |
author_sort |
Sess Tchotch, Didier Axel |
title |
Analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (PAHs) in cocoa butter using HPLC-FLD |
title_short |
Analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (PAHs) in cocoa butter using HPLC-FLD |
title_full |
Analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (PAHs) in cocoa butter using HPLC-FLD |
title_fullStr |
Analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (PAHs) in cocoa butter using HPLC-FLD |
title_full_unstemmed |
Analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (PAHs) in cocoa butter using HPLC-FLD |
title_sort |
analytical method validation and rapid determination of polycyclic aromatic hydrocarbons (pahs) in cocoa butter using hplc-fld |
url |
http://agritrop.cirad.fr/588508/ http://agritrop.cirad.fr/588508/7/n-durand-2018.pdf |
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