Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry
A new and convenient synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor compound in rice, and its ring-deuterated analog, 2-acetyl-1-d2-pyrroline (2AP-d2), was reported. A stable isotope dilution assay (SIDA), involving headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-positive chemical ionization-ion trap-tandem mass spectrometry (GC-PCI-ITMS- MS), was developed for 2AP quantification. A divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber was used for HS-SPME procedure and parameters affecting analytes recovery, such as extraction time and temperature, pH and salt, were studied. The repeatability of the method (n = 10) expressed as relative standard deviation (RSD) was 11.6%. A good linearity was observed from 5.9 to 779 ng of 2AP (r2 = 0.9989). Limits of detection (LOD) and quantification (LOQ) for 2AP were 0.1 and 0.4 ng g-1 of rice, respectively. The recovery of spiked 2AP from rice matrix was almost complete. The developed method was applied to the quantification of 2AP in aerial parts and grains of scented and non-scented rice cultivars.
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Subjects: | Q04 - Composition des produits alimentaires, U30 - Méthodes de recherche, riz, composé aromatique, biosynthèse, odeur, spectrométrie de masse, chromatographie, chromatographie en phase gazeuse, http://aims.fao.org/aos/agrovoc/c_6599, http://aims.fao.org/aos/agrovoc/c_622, http://aims.fao.org/aos/agrovoc/c_928, http://aims.fao.org/aos/agrovoc/c_14344, http://aims.fao.org/aos/agrovoc/c_12421, http://aims.fao.org/aos/agrovoc/c_1594, http://aims.fao.org/aos/agrovoc/c_11097, http://aims.fao.org/aos/agrovoc/c_6272, http://aims.fao.org/aos/agrovoc/c_3081, |
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dig-cirad-fr-5566682024-01-28T18:37:43Z http://agritrop.cirad.fr/556668/ http://agritrop.cirad.fr/556668/ Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry. Maraval Isabelle, Sen Kemal, Agrebi Abdelhamid, Menut Chantal, Morere Alain, Boulanger Renaud, Gay Frédéric, Mestres Christian, Günata Ziya. 2010. Analytica Chimica Acta, 675 (2) : 148-155.https://doi.org/10.1016/j.aca.2010.07.024 <https://doi.org/10.1016/j.aca.2010.07.024> Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry Maraval, Isabelle Sen, Kemal Agrebi, Abdelhamid Menut, Chantal Morere, Alain Boulanger, Renaud Gay, Frédéric Mestres, Christian Günata, Ziya eng 2010 Analytica Chimica Acta Q04 - Composition des produits alimentaires U30 - Méthodes de recherche riz composé aromatique biosynthèse odeur spectrométrie de masse chromatographie chromatographie en phase gazeuse http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_622 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_14344 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_11097 Provence-Alpes-Côte d'Azur France http://aims.fao.org/aos/agrovoc/c_6272 http://aims.fao.org/aos/agrovoc/c_3081 A new and convenient synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor compound in rice, and its ring-deuterated analog, 2-acetyl-1-d2-pyrroline (2AP-d2), was reported. A stable isotope dilution assay (SIDA), involving headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-positive chemical ionization-ion trap-tandem mass spectrometry (GC-PCI-ITMS- MS), was developed for 2AP quantification. A divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber was used for HS-SPME procedure and parameters affecting analytes recovery, such as extraction time and temperature, pH and salt, were studied. The repeatability of the method (n = 10) expressed as relative standard deviation (RSD) was 11.6%. A good linearity was observed from 5.9 to 779 ng of 2AP (r2 = 0.9989). Limits of detection (LOD) and quantification (LOQ) for 2AP were 0.1 and 0.4 ng g-1 of rice, respectively. The recovery of spiked 2AP from rice matrix was almost complete. The developed method was applied to the quantification of 2AP in aerial parts and grains of scented and non-scented rice cultivars. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/556668/1/document_556668.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.aca.2010.07.024 10.1016/j.aca.2010.07.024 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2010.07.024 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.aca.2010.07.024 |
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Q04 - Composition des produits alimentaires U30 - Méthodes de recherche riz composé aromatique biosynthèse odeur spectrométrie de masse chromatographie chromatographie en phase gazeuse http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_622 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_14344 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_6272 http://aims.fao.org/aos/agrovoc/c_3081 Q04 - Composition des produits alimentaires U30 - Méthodes de recherche riz composé aromatique biosynthèse odeur spectrométrie de masse chromatographie chromatographie en phase gazeuse http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_622 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_14344 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_6272 http://aims.fao.org/aos/agrovoc/c_3081 |
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Q04 - Composition des produits alimentaires U30 - Méthodes de recherche riz composé aromatique biosynthèse odeur spectrométrie de masse chromatographie chromatographie en phase gazeuse http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_622 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_14344 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_6272 http://aims.fao.org/aos/agrovoc/c_3081 Q04 - Composition des produits alimentaires U30 - Méthodes de recherche riz composé aromatique biosynthèse odeur spectrométrie de masse chromatographie chromatographie en phase gazeuse http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_622 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_14344 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_6272 http://aims.fao.org/aos/agrovoc/c_3081 Maraval, Isabelle Sen, Kemal Agrebi, Abdelhamid Menut, Chantal Morere, Alain Boulanger, Renaud Gay, Frédéric Mestres, Christian Günata, Ziya Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry |
description |
A new and convenient synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor compound in rice, and its ring-deuterated analog, 2-acetyl-1-d2-pyrroline (2AP-d2), was reported. A stable isotope dilution assay (SIDA), involving headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-positive chemical ionization-ion trap-tandem mass spectrometry (GC-PCI-ITMS- MS), was developed for 2AP quantification. A divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber was used for HS-SPME procedure and parameters affecting analytes recovery, such as extraction time and temperature, pH and salt, were studied. The repeatability of the method (n = 10) expressed as relative standard deviation (RSD) was 11.6%. A good linearity was observed from 5.9 to 779 ng of 2AP (r2 = 0.9989). Limits of detection (LOD) and quantification (LOQ) for 2AP were 0.1 and 0.4 ng g-1 of rice, respectively. The recovery of spiked 2AP from rice matrix was almost complete. The developed method was applied to the quantification of 2AP in aerial parts and grains of scented and non-scented rice cultivars. |
format |
article |
topic_facet |
Q04 - Composition des produits alimentaires U30 - Méthodes de recherche riz composé aromatique biosynthèse odeur spectrométrie de masse chromatographie chromatographie en phase gazeuse http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_622 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_14344 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_6272 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Maraval, Isabelle Sen, Kemal Agrebi, Abdelhamid Menut, Chantal Morere, Alain Boulanger, Renaud Gay, Frédéric Mestres, Christian Günata, Ziya |
author_facet |
Maraval, Isabelle Sen, Kemal Agrebi, Abdelhamid Menut, Chantal Morere, Alain Boulanger, Renaud Gay, Frédéric Mestres, Christian Günata, Ziya |
author_sort |
Maraval, Isabelle |
title |
Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry |
title_short |
Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry |
title_full |
Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry |
title_fullStr |
Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry |
title_full_unstemmed |
Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry |
title_sort |
quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry |
url |
http://agritrop.cirad.fr/556668/ http://agritrop.cirad.fr/556668/1/document_556668.pdf |
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