Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry
A new and convenient synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor compound in rice, and its ring-deuterated analog, 2-acetyl-1-d2-pyrroline (2AP-d2), was reported. A stable isotope dilution assay (SIDA), involving headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-positive chemical ionization-ion trap-tandem mass spectrometry (GC-PCI-ITMS- MS), was developed for 2AP quantification. A divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber was used for HS-SPME procedure and parameters affecting analytes recovery, such as extraction time and temperature, pH and salt, were studied. The repeatability of the method (n = 10) expressed as relative standard deviation (RSD) was 11.6%. A good linearity was observed from 5.9 to 779 ng of 2AP (r2 = 0.9989). Limits of detection (LOD) and quantification (LOQ) for 2AP were 0.1 and 0.4 ng g-1 of rice, respectively. The recovery of spiked 2AP from rice matrix was almost complete. The developed method was applied to the quantification of 2AP in aerial parts and grains of scented and non-scented rice cultivars.
| Main Authors: | , , , , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | Q04 - Composition des produits alimentaires, U30 - Méthodes de recherche, riz, composé aromatique, biosynthèse, odeur, spectrométrie de masse, chromatographie, chromatographie en phase gazeuse, http://aims.fao.org/aos/agrovoc/c_6599, http://aims.fao.org/aos/agrovoc/c_622, http://aims.fao.org/aos/agrovoc/c_928, http://aims.fao.org/aos/agrovoc/c_14344, http://aims.fao.org/aos/agrovoc/c_12421, http://aims.fao.org/aos/agrovoc/c_1594, http://aims.fao.org/aos/agrovoc/c_11097, http://aims.fao.org/aos/agrovoc/c_6272, http://aims.fao.org/aos/agrovoc/c_3081, |
| Online Access: | http://agritrop.cirad.fr/556668/ http://agritrop.cirad.fr/556668/1/document_556668.pdf |
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