Capillary electrophoresis-mass spectrometry for peptide analysis: Target-based approaches and proteomics/ peptidomics strategies
In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for peptide analysis is demonstrated by the presentation of two different strategies typically followed in analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic oligopeptide in a complex matrix. Namely, CE-MS using an ion trap MS analyzer is applied to detect and quantify γ-glutamyl-S-ethenyl-cysteine (GEC) bioactive dipeptide in a legume plant. The second one is a shotgunlike methodology used for proteomic analysis. Particularly, CE-MS using a time-of- flight MS analyzer is employed to investigate the substantial equivalence between a genetically modified (GM) variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics. © Springer Science+Business Media, LLC 2013.
| Main Authors: | , , |
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| Other Authors: | |
| Format: | capítulo de libro biblioteca |
| Published: |
Humana Press
2013
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| Subjects: | CE-MS, CE, Target analysis, Proteomics, Peptidomics, Foodomics, Peptides, |
| Online Access: | http://hdl.handle.net/10261/99820 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100004240 |
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