Isolation, identification and characterization of Lactobacillus species from fermented locust bean (Parkia biglobosa) for use as probiotics in the aquaculture industry
Probiotics are a promising feed additive to stimulate animal growth and secure a low disease response in aquaculture industry where there are high stocking densities in shrimp and fish production. Fermented locust beans (Parkia biglobosa) are known to be rich in protein and used as food condiment. Probiotic bacteria were isolated from this locally available food material. Culture and characterizations of isolates were carried out. Sugar fermentation patterns were determined by using an API 50 CHL system and incubation were performed anaerobically at 37~'C. MRS broth culture grown at 37~'C overnight was added to 9ml of MRS Agar and the bacteria were incubated at 37~'C for 24 and 48 hrs. Discrete and single colony of lactobacillus was isolated using colony morphology and biochemical tests. The most significant viable taxa isolated was Lactobacillus fermentum at a pH range of (3.0-8.0), while the least viable taxa isolated was Leuconostoc mesenteroides ssp. Microscopically they were Grampositive, rod shaped, non- motile, catalase negative and absence of Endospore.
| Main Authors: | , |
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| Format: | conference_item biblioteca |
| Language: | English |
| Published: |
FISON
2012
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| Subjects: | Aquaculture, Nigeria, Lagos, Locust bean, Probiotics, Lactobacillus, freshwater environment, Feed, Additives, Microorganisms, Bacteria, Disease resistance, Fermented products, Fish culture, Shrimp culture, |
| Online Access: | http://hdl.handle.net/1834/38856 |
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