Extraction and determination of PSP (saxitoxin and derivatives) from scallop
Paralytic Shellfish Poison (saxitoxin and derivatives) was extracted by HCL method from frozen scallops. The samples were collected from coastal areas of Iwate prefecture- Japan. Toxicity of the samples was determined with mouse bioassay method. Intra peritoneal injection was done to dry strain 19-21 grams male mouse, and death time was listed. In conclusion, 33.80, 38.40, 31.20 MU/g (or 777, 883, 718 STX/l00gr) were obtained respectively as the toxicity of the samples of three different sampling areas. The toxicity for the areas was 9.7, 11 and 9 times more than standard level (80STX/100g) and very dangerous for human consumption. The kind of toxin was determined by comparing HPLC graph of sample with standard saxitoxin HPLC graph.
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| Main Author: | |
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| Format: | article biblioteca |
| Language: | Persian |
| Published: |
2007
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| Subjects: | Biology, Bioassays, Biological poisons, Toxicity, Marine, HPLC, Human diseases, Paralytic shellfish poisoning, Pectinidae, ANW, Japan, |
| Online Access: | http://hdl.handle.net/1834/38347 |
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