Soybean B-Glucosidase immobilisated on chitosan beads and its applicaation in soy drink increase the Aglycones.

The objective of this study was to investigate the immobilisation efficiency of soybean β-glucosidase (181.6 U/mL; 23.8 mg protein/mL) on activated chitosan beads. Central Composite Rotational Design (CCDR) 2 was used and the application of immobilised enzyme in commercial soy drink was evaluated. The activation of chitosan beads was achieved with established 2.5% glutaraldehyde, pH 7.5, 8 h incubation time (6 h with agitation and 2 h without agitation) at 37ºC. The highest immobilisation efficiency (%) of soybean β-glucosidase on chitosan beads obtained was 37.74 U/mL and 18.84 mg protein/4 chitosan beads at pH 7.5 and 20 h coupling time of enzyme-matrix (7 h with agitation and 13 h without agitation) at 4ºC. The immobilised enzyme incubated at 50ºC, pH 5.5 resulted in 24% increase in the aglycones content in commercial soy drink after 60 min.

Bibliographic Details

Main Authors:	GRADE, L. C., MOREIRA, A. A., VAREA, G. da S., MANDARINO, J. M. G., SILVA, J. B. da, IDA, E. I., RIBEIRO, M. L. L.
Other Authors:	LUCIANA CARVALHO GRADE, UEL; AMANDA ALEIXO MOREIRA, UEL; GENI DA SILVA VAREA, UEL; JOSÉ MARCOS GONTIJO MANDARINO, CNPSO; JOSEMEYRE BONIFÁCIO DA SILVA, UEL; ELZA IOUKO IDA, UEL; MARA LÚCIA LUIZ RIBEIRO, UEL.
Format:	Artigo de periódico biblioteca
Language:	pt_BR por
Published:	2014-09-03
Subjects:	Soja., Soybeans.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/994090 http://dx.doi.org/10.1590/S1516-8913201402331
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