Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a

by Olivi, Lorenzo, Bagchus, Cleo, Pool, Victor, Bekkering, Ezra, Speckner, Konstantin, Offerhaus, Hidde, Wu, Wen Y., Depken, Martin, Martens, Koen, Staals, Raymond, Hohlbein, Johannes

Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types

by Wu, Wen Y., Jackson, Simon A., Almendros, Cristóbal, Haagsma, Anna C., Yilmaz, Suzan, Gort, Gerrit, van der Oost, John, Brouns, Stan J.J., Staals, Raymond H.J.

Mechanistic and evolutionary insights into a type V-M CRISPR–Cas effector enzyme

by Omura, Satoshi N., Nakagawa, Ryoya, Südfeld, Christian, Villegas Warren, Ricardo, Wu, Wen Y., Hirano, Hisato, Laffeber, Charlie, Kusakizako, Tsukasa, Kise, Yoshiaki, Lebbink, Joyce H.G., Itoh, Yuzuru, van der Oost, John, Nureki, Osamu

The miniature CRISPR-Cas12m effector binds DNA to block transcription

by Wu, Wen Y., Mohanraju, Prarthana, Liao, Chunyu, Adiego-Pérez, Belén, Creutzburg, Sjoerd C.A., Makarova, Kira S., Keessen, Karlijn, Lindeboom, Timon A., Khan, Tahseen S., Prinsen, Stijn, Joosten, Rob, Yan, Winston X., Migur, Anzhela, Laffeber, Charlie, Scott, David A., Lebbink, Joyce H.G., Koonin, Eugene V., Beisel, Chase L., van der Oost, John

Cryo-EM structure of the transposon-associated TnpB enzyme

by Nakagawa, Ryoya, Hirano, Hisato, Omura, Satoshi N., Nety, Suchita, Kannan, Soumya, Altae-Tran, Han, Yao, Xiao, Sakaguchi, Yuriko, Ohira, Takayuki, Wu, Wen Y., Nakayama, Hiroshi, Shuto, Yutaro, Tanaka, Tatsuki, Sano, Fumiya K., Kusakizako, Tsukasa, Kise, Yoshiaki, Itoh, Yuzuru, Dohmae, Naoshi, van der Oost, John, Suzuki, Tsutomu, Zhang, Feng, Nureki, Osamu