Cytogenetic studies in four cultivated Amaranthus (Amaranthaceae) species
In the present study, the chromosomes numbers were confirmed, 2n = 34 for Amaranthus cruentus Linnaeus, 1759, and 2n = 32 for A. hypochondriacus Linnaeus, 1753, A. mantegazzianus Passer, 1864, and A. caudatus Linnaeus, 1753. The distribution and variability of constitutive heterochromatin were detailed using DAPI-CMA3 banding technique. The position of the nucleolus organizer region (NOR) was observed using Ag-NOR banding (active loci) and fluorescent in situ hybridization (rDNA-FISH) in the four Amaranthus species. Variations in the amount of constitutive heterochromatin were detected both within the species and between them, with DAPI-CMA3 stain. One chromosome pair having a NOR was found in each studied accession, with exception of A. caudatus cv. EEA INTA Anguil. This accession presented four rDNA loci (FISH), being active two of them (Ag-banding).
| Main Authors: | , , |
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| Format: | info:eu-repo/semantics/article biblioteca |
| Language: | eng |
| Subjects: | AMARANTHUS, CONSTITUTIVE HETEROCHROMATIN, DAPI-CMA3, FISH, NORS, |
| Online Access: | http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2013bonasora |
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| Summary: | In the present study, the chromosomes numbers were confirmed, 2n = 34 for Amaranthus cruentus Linnaeus, 1759, and 2n = 32 for A. hypochondriacus Linnaeus, 1753, A. mantegazzianus Passer, 1864, and A. caudatus Linnaeus, 1753. The distribution and variability of constitutive heterochromatin were detailed using DAPI-CMA3 banding technique. The position of the nucleolus organizer region (NOR) was observed using Ag-NOR banding (active loci) and fluorescent in situ hybridization (rDNA-FISH) in the four Amaranthus species. Variations in the amount of constitutive heterochromatin were detected both within the species and between them, with DAPI-CMA3 stain. One chromosome pair having a NOR was found in each studied accession, with exception of A. caudatus cv. EEA INTA Anguil. This accession presented four rDNA loci (FISH), being active two of them (Ag-banding). |
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