Direct transfer of frozen bovine embryos in one step
The methodology to simplify the freezing-thawing and implantation method of bovine embryos is described. For freezing, a buffer solution (Dulbecco) with ethylene glycol as cryoprotectant is used, plus the addition of bovine serum albumin at 4% weight/volume. Defrosting is done 5 seconds in the air and then in a water bath at 25ºC for 20 seconds. The implantation is immediately carried out with extreme delicacy given the fragility of the biological material. The authors report 8 pregnancies out of 17 embryos implanted with this system, considering it satisfactory but perfectible.
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| Main Authors: | , , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Sociedad de Medicina Veterinaria del Uruguay (SMVU)
1998
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| Online Access: | https://www.revistasmvu.com.uy/index.php/smvu/article/view/511 |
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| Summary: | The methodology to simplify the freezing-thawing and implantation method of bovine embryos is described. For freezing, a buffer solution (Dulbecco) with ethylene glycol as cryoprotectant is used, plus the addition of bovine serum albumin at 4% weight/volume. Defrosting is done 5 seconds in the air and then in a water bath at 25ºC for 20 seconds. The implantation is immediately carried out with extreme delicacy given the fragility of the biological material. The authors report 8 pregnancies out of 17 embryos implanted with this system, considering it satisfactory but perfectible. |
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