First molecular studies in the dog Cimarrón del Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development
The Cimarrón dog is the only canine breed native in Uruguay. In a conservation program of this breed is important to use molecular DNA markers named RAPD to know the genetic variability. The objective of this work is to evaluate a series of 10 RAPD markers in DNA samples of Cimarrón dogs with the PCR methodology. There were loci amplification in 70% of the markers. RAPD 401 and 403 had a higher number of polimorphic loci (58.33%), with a band of sharing frequency of S=0.79 and 0.86 respectively. This numbers would indicate us a genomic homogenity in the analized sample. The use of this RAPD series in a higher Cimarrón dog sample like in other breeds will let us do comparative studies of variability in populations.
Main Authors: | , , , |
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Format: | Digital revista |
Language: | spa |
Published: |
Sociedad de Medicina Veterinaria del Uruguay (SMVU)
2004
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Online Access: | https://www.revistasmvu.com.uy/index.php/smvu/article/view/497 |
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Summary: | The Cimarrón dog is the only canine breed native in Uruguay. In a conservation program of this breed is important to use molecular DNA markers named RAPD to know the genetic variability. The objective of this work is to evaluate a series of 10 RAPD markers in DNA samples of Cimarrón dogs with the PCR methodology. There were loci amplification in 70% of the markers. RAPD 401 and 403 had a higher number of polimorphic loci (58.33%), with a band of sharing frequency of S=0.79 and 0.86 respectively. This numbers would indicate us a genomic homogenity in the analized sample. The use of this RAPD series in a higher Cimarrón dog sample like in other breeds will let us do comparative studies of variability in populations. |
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