Evaluation of cane molasses as substrate for Lactobacillus plantarum growth
Due the importance of lactic acid bacteria (LAB) in Colombia and worldwide, it has been necessary to optimize the culture conditions using different kind of substrates to increase growth. In this research, cane molasses was exploited as an indicator substrate to determinate Lactobacillus plantarum growth. Different conditions and variables that affect the microbial development were evaluated L. plantarum; (WS417) was used as a control strain, inoculating diverse sterile molasses concentrations at different pH values, temperature and agitation. The strain growth was evaluated by traditional microbiology, direct bacteria count, seeded in MRS agar plates. Relationships between biomass and effectiveness of different culture conditions were estimated by factorial variance analysis, showing the results as optimal growth conditions the content of 20% cane molasses, 30±1°C for 24 hours, pH 5,2±0,1, and 100rpm with counts of 43x109UFC/mL. It was concluded that cane molasses could be employed as growth substrate for Lactobacillus sp.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
2010
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| Online Access: | https://revistas.udca.edu.co/index.php/ruadc/article/view/713 |
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| Summary: | Due the importance of lactic acid bacteria (LAB) in Colombia and worldwide, it has been necessary to optimize the culture conditions using different kind of substrates to increase growth. In this research, cane molasses was exploited as an indicator substrate to determinate Lactobacillus plantarum growth. Different conditions and variables that affect the microbial development were evaluated L. plantarum; (WS417) was used as a control strain, inoculating diverse sterile molasses concentrations at different pH values, temperature and agitation. The strain growth was evaluated by traditional microbiology, direct bacteria count, seeded in MRS agar plates. Relationships between biomass and effectiveness of different culture conditions were estimated by factorial variance analysis, showing the results as optimal growth conditions the content of 20% cane molasses, 30±1°C for 24 hours, pH 5,2±0,1, and 100rpm with counts of 43x109UFC/mL. It was concluded that cane molasses could be employed as growth substrate for Lactobacillus sp. |
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