Sequencing of new bola-DRB3.2 alleles detected in mexican Creole cattle
The nucleic acid sequence of ten BoLA-DRB3.2 alleles detected by PCR-RFLP in Mexican Creole cattle was determined. The methodology used to determine such sequence was the SBT (sequence based typing). It was possible to determine that two alleles, correspond to DRB3*1602 and DRB3*1501 previously reported to the BoLA database. The remaining eight alleles had nucleic acid sequences different from those already published. When compared to the officially reported alleles, three of those defined in this study showed maximal homologies of 99 %, four had 98 % homology and one showed maximal identity of 94 %, therefore the conclusion was that those 8 alleles are unreported. The amino acid sequences deduced for these alleles showed differences from 2 to 51 % with respect to the officially published peptides. The sequence analysis of these new alleles supports the hypothesis that Mexican creole cattle possibly has a higher genetic potential to respond to a wider range of pathogen antigens than the other bovine races studied to date.
| Main Authors: | , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2012
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| Online Access: | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1763 |
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| Summary: | The nucleic acid sequence of ten BoLA-DRB3.2 alleles detected by PCR-RFLP in Mexican Creole cattle was determined. The methodology used to determine such sequence was the SBT (sequence based typing). It was possible to determine that two alleles, correspond to DRB3*1602 and DRB3*1501 previously reported to the BoLA database. The remaining eight alleles had nucleic acid sequences different from those already published. When compared to the officially reported alleles, three of those defined in this study showed maximal homologies of 99 %, four had 98 % homology and one showed maximal identity of 94 %, therefore the conclusion was that those 8 alleles are unreported. The amino acid sequences deduced for these alleles showed differences from 2 to 51 % with respect to the officially published peptides. The sequence analysis of these new alleles supports the hypothesis that Mexican creole cattle possibly has a higher genetic potential to respond to a wider range of pathogen antigens than the other bovine races studied to date. |
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