Pseudorecombination of Potato Yellow Mosaic Virus with Begomoviruses Isolated from Weeds
In mixed infections between begomoviruses, common in tropical and subtropical regions, events such as transcomplementation and transreplication can occur. The aim of this research was to evaluate the possibility of asymmetric transreplication and transcomplementation events between Potato yellow mosaic virus (PYMV) and three begomoviruses previously isolated from tomato crop-associated weeds: Croton golden mosaic virus (CohGMV), Rhynchosia golden mosaic Colombia virus (RhGMCV) and Verbena mottle virus (VeMV). To achieve this goal, tomato leaves were inoculated with artificial mixtures of genomic components from PYMV, CohGMV, RhGMCV, or VeMV by using biolistic. The possible interactions between the viruses at the level of replication, movement and symptom expression were analyzed. Our results showed asymmetric complementation at the level of movement and replication, as well as heterologous transactivation events in mixed infections between PYMV with CohGMV, RhGMCV, or VeMV. To explain the possible asymmetrical complementation at the level of movement and replication between these begomoviruses, a bioinformatic analysis of the viral promoters of PYMV, CohGMV, RhGMCV and VeMV was performed. Similarities in cis-regulatory elements of the analyzed regions in the begomovirus studied were identified in our analysis. Asymmetrical complementation and transactivation events observed under experimental conditions suggest that if mixed infections occur under natural field conditions, new pseudorecombinats could emerge.
| Main Authors: | , , |
|---|---|
| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
2023
|
| Online Access: | https://revistas.unal.edu.co/index.php/actabiol/article/view/98952 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | In mixed infections between begomoviruses, common in tropical and subtropical regions, events such as transcomplementation and transreplication can occur. The aim of this research was to evaluate the possibility of asymmetric transreplication and transcomplementation events between Potato yellow mosaic virus (PYMV) and three begomoviruses previously isolated from tomato crop-associated weeds: Croton golden mosaic virus (CohGMV), Rhynchosia golden mosaic Colombia virus (RhGMCV) and Verbena mottle virus (VeMV). To achieve this goal, tomato leaves were inoculated with artificial mixtures of genomic components from PYMV, CohGMV, RhGMCV, or VeMV by using biolistic. The possible interactions between the viruses at the level of replication, movement and symptom expression were analyzed. Our results showed asymmetric complementation at the level of movement and replication, as well as heterologous transactivation events in mixed infections between PYMV with CohGMV, RhGMCV, or VeMV. To explain the possible asymmetrical complementation at the level of movement and replication between these begomoviruses, a bioinformatic analysis of the viral promoters of PYMV, CohGMV, RhGMCV and VeMV was performed. Similarities in cis-regulatory elements of the analyzed regions in the begomovirus studied were identified in our analysis. Asymmetrical complementation and transactivation events observed under experimental conditions suggest that if mixed infections occur under natural field conditions, new pseudorecombinats could emerge. |
|---|