Germoplasm ex situ conservation and in vitro propagation of Neltuma alba Griseb
Neltuma alba Gris is an emblematic specie of Chaco Ecoregion for its wood and fruits value. This research show that algarrobo blanco´s seeds can cryopreserve without affecting germination power and energy. The seeds were kept for three months at 24°-30°C, (-18°C) and (-196°C). Later, they were mechanically scarified and sown in black soil and sawdust (3: 1). The results were subjected to non-parametric Kruskal-Wallis tests, analyzing germination power, energy and vigor. The test did not detect significant differences between treatments. The nodal segments (2 cm long) obtained from plants of seeds were used for in vitro regeneration in MS culture medium supplemented with 3 mg L-1 IBA and 0.05 mg L-1 CIN, The rooting percentages obtained were high (87-90%). There are no significant differences between treatments tested. The storage of seeds at 24°-30°C is the most approppiate to rich high percentages of germination.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología
2022
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| Online Access: | https://revistas.unal.edu.co/index.php/biotecnologia/article/view/97900 |
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| Summary: | Neltuma alba Gris is an emblematic specie of Chaco Ecoregion for its wood and fruits value. This research show that algarrobo blanco´s seeds can cryopreserve without affecting germination power and energy.
The seeds were kept for three months at 24°-30°C, (-18°C) and (-196°C). Later, they were mechanically scarified and sown in black soil and sawdust (3: 1). The results were subjected to non-parametric Kruskal-Wallis tests, analyzing germination power, energy and vigor. The test did not detect significant differences between treatments.
The nodal segments (2 cm long) obtained from plants of seeds were used for in vitro regeneration in MS culture medium supplemented with 3 mg L-1 IBA and 0.05 mg L-1 CIN, The rooting percentages obtained were high (87-90%). There are no significant differences between treatments tested.
The storage of seeds at 24°-30°C is the most approppiate to rich high percentages of germination. |
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