Detección de las infecciones congénitas y persistentes por el virus de la peste porcina clásica
Persistent infections and congenital disease caused by Classical Swine Fever Virus (CSFV) were studied using RT-nested PCR. For this purpose, tissues samples collected from positive field cases, tonsils from discarded gestating sows with their litter and other tonsils also from discarded sows were examined. In order to detect the viral nucleic acid a set of oligonucleotides primers directed to E2 gene were used to amplify a fragment of 270pb. Then, those amplified were cut with restriction enzymes to generate RFLP’s. Positives samples were antigenically studied by using monoclonal antibodies conjugates with peroxidase. The fragment belonged to E2 gene was amplified in 14 out of 20 field cases analyzed. Similarly, the viral genome was also detected in 8 tonsils sows samples, 6 of which belonged to gestating sows and the other 2 to discarded sows. The positive samples by RT-PCR were classified as field strains by monoclonal antibody. The PCR amplified were grouped within group 4 using the Avall enzyme and within group 1 by digestión with enzyme PvuII. It noticed a partial digestión when using BanII enzyme. The epidemiológical significance of these findings is discussed.
| Main Authors: | , , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina Veterinaria y de Zootecnia
2004
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| Online Access: | https://revistas.unal.edu.co/index.php/remevez/article/view/94688 |
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| Summary: | Persistent infections and congenital disease caused by Classical Swine Fever Virus (CSFV) were studied using RT-nested PCR. For this purpose, tissues samples collected from positive field cases, tonsils from discarded gestating sows with their litter and other tonsils also from discarded sows were examined. In order to detect the viral nucleic acid a set of oligonucleotides primers directed to E2 gene were used to amplify a fragment of 270pb. Then, those amplified were cut with restriction enzymes to generate RFLP’s. Positives samples were antigenically studied by using monoclonal antibodies conjugates with peroxidase. The fragment belonged to E2 gene was amplified in 14 out of 20 field cases analyzed. Similarly, the viral genome was also detected in 8 tonsils sows samples, 6 of which belonged to gestating sows and the other 2 to discarded sows. The positive samples by RT-PCR were classified as field strains by monoclonal antibody. The PCR amplified were grouped within group 4 using the Avall enzyme and within group 1 by digestión with enzyme PvuII. It noticed a partial digestión when using BanII enzyme. The epidemiológical significance of these findings is discussed. |
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