Regeneration Potential of Psychotria Ipecacuanha (Rubiaceae) from Thin Cell Layers
Knowing the medicinal properties of the plant specie Psychotria ipecacuanha (Brot.) Stokes, its critical condition and the difficulties for its effective propagation, the present study aimed to assess the potential of propagation of P. ipecacuanha by in vitro systems of regeneration, organogenesis and somatic embryogenesis. For this purpose, thin cell layers (TCL) of stems and leaves, as well as leaf segments were subjected to different treatments of plant growth regulators and light conditions. Furthermore, the effect of different wavelengths via light emitting diodes (LED’s), was established for the regeneration in these explants and nodal explants from in vitro plants. The results showed that leaf segments and stem TCL, cultured in MS medium supplemented with the combinations of growth regulators IBA + BAP and IBA + TDZ, formed somatic embryos and shoots. The histological sections supported the two types of source. It was found that the best results in shoot induction and embryos were achieved under the light condition 16/8-h light/dark. Regarding the effect of different wavelengths, it was found that those corresponding to red, green, and white supported the growth and shoot development as well as somatic embryos induction. The shoots development from the nodal explants did not show statistical differences between LEDs treatments, so the use of a continuous white light and photoperiod is recommended during their multiplication and development.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
2015
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| Online Access: | https://revistas.unal.edu.co/index.php/actabiol/article/view/47354 |
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| Summary: | Knowing the medicinal properties of the plant specie Psychotria ipecacuanha (Brot.) Stokes, its critical condition and the difficulties for its effective propagation, the present study aimed to assess the potential of propagation of P. ipecacuanha by in vitro systems of regeneration, organogenesis and somatic embryogenesis. For this purpose, thin cell layers (TCL) of stems and leaves, as well as leaf segments were subjected to different treatments of plant growth regulators and light conditions. Furthermore, the effect of different wavelengths via light emitting diodes (LED’s), was established for the regeneration in these explants and nodal explants from in vitro plants. The results showed that leaf segments and stem TCL, cultured in MS medium supplemented with the combinations of growth regulators IBA + BAP and IBA + TDZ, formed somatic embryos and shoots. The histological sections supported the two types of source. It was found that the best results in shoot induction and embryos were achieved under the light condition 16/8-h light/dark. Regarding the effect of different wavelengths, it was found that those corresponding to red, green, and white supported the growth and shoot development as well as somatic embryos induction. The shoots development from the nodal explants did not show statistical differences between LEDs treatments, so the use of a continuous white light and photoperiod is recommended during their multiplication and development. |
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