Depth-scanning confocal Raman for rapid in vivo determination of testosterone concentration profiles in human skin

Depth-scanning confocal Raman for rapid in vivo determination of testosterone concentration profiles in human skin

OBJECTIVE: The present study aimed to measure in-vivo the depth and the percentage of testosterone in human skin. METHOD: Two healthy young Brazilian volunteers were evaluated through a Confocal Raman Spectroscopy probe on the right volar forearm. Testosterone spectroscopy of was performed on the Stratum Corneum, Viable Epidermis and Dermis; the percentage concentration of testosterone was compared between the baseline and one hour after local application of a transdermal nanostructured testosterone (5%) formulation. RESULTS: To the best of our knowledge, this is the first time that the depth and percentage of testosterone has been evaluated non-invasively in-vivo. No adverse events were attributed to this protocol. The method is effective in differentially measuring testosterone in the skin layers. CONCLUSION: This protocol may serve as a good choice for rapid hormone quantification for forensic or medical purposes.

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Bibliographic Details
Main Authors: Botelho,Marco Antonio, Guerreiro,Stela Julia, Queiroz,Dinalva Brito, Barros,Gisele, Cavalcante,Monaliza, Souza,Juliana M. O., Silva,André M., Lemos,Telma L. G., Quintans Jr,Lucindo
Format: Digital revista
Language:English
Published: Mavera Edições Técnicas e Científicas Ltda 2014
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2358-04292014000100031
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Summary:OBJECTIVE: The present study aimed to measure in-vivo the depth and the percentage of testosterone in human skin. METHOD: Two healthy young Brazilian volunteers were evaluated through a Confocal Raman Spectroscopy probe on the right volar forearm. Testosterone spectroscopy of was performed on the Stratum Corneum, Viable Epidermis and Dermis; the percentage concentration of testosterone was compared between the baseline and one hour after local application of a transdermal nanostructured testosterone (5%) formulation. RESULTS: To the best of our knowledge, this is the first time that the depth and percentage of testosterone has been evaluated non-invasively in-vivo. No adverse events were attributed to this protocol. The method is effective in differentially measuring testosterone in the skin layers. CONCLUSION: This protocol may serve as a good choice for rapid hormone quantification for forensic or medical purposes.

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