Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
2007
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400015 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines. |
|---|