Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR

Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR

Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.

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Bibliographic Details
Main Authors: Figueiredo,Eduardo Eustáquio de Souza, Carvalho,Ricardo Cezar Tavares, Silvestre,Flávia Galindo, Lilenbaum,Walter, Fonseca,Leila Sousa, Silva,Joab Trajano, Paschoalin,Vânia Margaret Flosi
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 2010
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020
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Summary:Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.

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