Performance of an immunoenzymatic assay for Cryptosporidium diagnosis of fecal samples

Performance of an immunoenzymatic assay for Cryptosporidium diagnosis of fecal samples

We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a specificity of 96%; positive and negative predictive values were 89% and 100%, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.

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Bibliographic Details
Main Authors: Marques,Fabiana Rangel, Cardoso,Luciana Ventura, Cavasini,Carlos Eugênio, Almeida,Magali Carmem de, Bassi,Nair Aparecida, Almeida,Margarete Teresa Gottardo de, Rossit,Andréa Regina Baptista, Machado,Ricardo Luiz Dantas
Format: Digital revista
Language:English
Published: Brazilian Society of Infectious Diseases 2005
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100002
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Summary:We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a specificity of 96%; positive and negative predictive values were 89% and 100%, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.

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