Total phenolic compounds, flavonoids and antioxidant activity in the flowers of Crataegus spp. from México
There are a number of medicinally active phytochemicals isolated from hawthorn with most of the data generated in studies of those species that are native to Europe and Asia. Comparatively little is known about the North American, specifically the Mexican Crataegus species. The objective of this study was to evaluate phenolic compounds, flavonoids and antioxidant activity of extracts of flowers from six Mexican accessions of Crataegus in order to contribute to the chemistry of the Germplasm Bank accessions at the Universidad Autonóma Chapingo. Antioxidant activity was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and the flavonoids were identified by HPLC-MS. Quercetin- 3-O-glucoside, quercetin-3-O-rhamnoside, quercetin 3-O-rhamnosyl-(1<img border=0 src="../../../../../img/revistas/agro/v46n7/a2s1.jpg">6)-glucoside and quercetin 3-O-rhamnosyl-(1<img border=0 src="../../../../../img/revistas/agro/v46n7/a2s1.jpg">2)-[rhamnosyl-(1<img border=0 src="../../../../../img/revistas/agro/v46n7/a2s1.jpg">6)]-glucoside were identified. Using the DPPH radical scavenging capacity assay, accessions 52 and 77 showed the greatest antioxidant activity (IC50=431.1±27.2 and 555.4±8.0 µg mL-1). Only flavonoids glycosides were identified in all six species and the phenolics content was higher than flavonoids. There were not found differences in the phenolics total content.
| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Colegio de Postgraduados
2012
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| Online Access: | http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952012000700002 |
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| Summary: | There are a number of medicinally active phytochemicals isolated from hawthorn with most of the data generated in studies of those species that are native to Europe and Asia. Comparatively little is known about the North American, specifically the Mexican Crataegus species. The objective of this study was to evaluate phenolic compounds, flavonoids and antioxidant activity of extracts of flowers from six Mexican accessions of Crataegus in order to contribute to the chemistry of the Germplasm Bank accessions at the Universidad Autonóma Chapingo. Antioxidant activity was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and the flavonoids were identified by HPLC-MS. Quercetin- 3-O-glucoside, quercetin-3-O-rhamnoside, quercetin 3-O-rhamnosyl-(1<img border=0 src="../../../../../img/revistas/agro/v46n7/a2s1.jpg">6)-glucoside and quercetin 3-O-rhamnosyl-(1<img border=0 src="../../../../../img/revistas/agro/v46n7/a2s1.jpg">2)-[rhamnosyl-(1<img border=0 src="../../../../../img/revistas/agro/v46n7/a2s1.jpg">6)]-glucoside were identified. Using the DPPH radical scavenging capacity assay, accessions 52 and 77 showed the greatest antioxidant activity (IC50=431.1±27.2 and 555.4±8.0 µg mL-1). Only flavonoids glycosides were identified in all six species and the phenolics content was higher than flavonoids. There were not found differences in the phenolics total content. |
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