A fast and efficient method for total DNA extraction from soil filamentous fungi
Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.
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| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Universidad de Tarapacá. Facultad de Ciencias Agronómicas
2014
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292014000200010 |
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| Summary: | Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective. |
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