HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS
1. The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C18 ODS Hypersil column of 250 ÷ 4.6 mm id with 5 ìçé particle size using simple mobile phase of methanol: water (1:1, v/v) at a flow rate of 1.0 mL min¹ and detected at 204 nm. The limit of detection of etoposide was 0.03 µg mL-1. The proposed method was found linear in the range of 0.25 µg mL¹ to 62.5 µg mL¹ and CV was 1.3% at 0.25 µg mL¹ , 0.48% at 3.90 µg mL¹ and 0.13% at 62.5 µg mL¹. The accuracy of the developed method for two injectable dosage forms was 99.4 and 99.7% with RSD of 0.3%. The validation results and statistical data demonstrate that the method is simple, cost effective, reproducible and suitable in quality analysis of the active ingredient and dosage form.
| Main Authors: | , , , , , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Sociedad Chilena de Química
2011
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400012 |
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| Summary: | 1. The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C18 ODS Hypersil column of 250 ÷ 4.6 mm id with 5 ìçé particle size using simple mobile phase of methanol: water (1:1, v/v) at a flow rate of 1.0 mL min¹ and detected at 204 nm. The limit of detection of etoposide was 0.03 µg mL-1. The proposed method was found linear in the range of 0.25 µg mL¹ to 62.5 µg mL¹ and CV was 1.3% at 0.25 µg mL¹ , 0.48% at 3.90 µg mL¹ and 0.13% at 62.5 µg mL¹. The accuracy of the developed method for two injectable dosage forms was 99.4 and 99.7% with RSD of 0.3%. The validation results and statistical data demonstrate that the method is simple, cost effective, reproducible and suitable in quality analysis of the active ingredient and dosage form. |
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