BIOSENSORS' PREPARATION TO BE USED FOR ENTRAPMENT METHOD WITH COPOLYMERS POLYANILINE DERIVATIVES
The entrapment method was used to prepare electrochemical biosensors. Using controlled potential method, copolymers were synthesized between aniline y sulfonated-anilines in aqueous media at pH 4.9 on Platinum disc. The copolymers were characterized by elemental analysis and spectroscopy. Glucose-oxidase was entrapped in the electropolymerization process, at pH 4.9 in aqueous media. The copolymers modified electrodes and copolymer/enzyme were studied as working electrodes for the oxidation of different H2O2 concentrations and indirect D-glucose quantification at pH 7, using controlled potential and the cyclic voltammetry methods (CV) correspondingly. A linear relationship between the hydrogen peroxide (or D-glucose) concentration and the electrochemical response was obtained at pH 7. The modified-electrodes displayed a fast time of currentresponse, limitedbythe cyclenumbers of the cyclic voltammetry, 232 s, andenough stability to allow for the measurements to be taken after several days.
| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Sociedad Chilena de Química
2010
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000200019 |
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| Summary: | The entrapment method was used to prepare electrochemical biosensors. Using controlled potential method, copolymers were synthesized between aniline y sulfonated-anilines in aqueous media at pH 4.9 on Platinum disc. The copolymers were characterized by elemental analysis and spectroscopy. Glucose-oxidase was entrapped in the electropolymerization process, at pH 4.9 in aqueous media. The copolymers modified electrodes and copolymer/enzyme were studied as working electrodes for the oxidation of different H2O2 concentrations and indirect D-glucose quantification at pH 7, using controlled potential and the cyclic voltammetry methods (CV) correspondingly. A linear relationship between the hydrogen peroxide (or D-glucose) concentration and the electrochemical response was obtained at pH 7. The modified-electrodes displayed a fast time of currentresponse, limitedbythe cyclenumbers of the cyclic voltammetry, 232 s, andenough stability to allow for the measurements to be taken after several days. |
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