Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth
We analyzed the effect of the combination of citral, eugenol and thymol, respectively the main constituents of essential oils of Cympobogon citratus (DC) Stapf, Poaceae (lemon grass), Syzygium aromaticum(L.) Merr. & L.M. Perry, Myrtaceae (clove) and Thymus vulgarisL., Lamiaceae (thyme), on the proliferation of the trypanosomatids Crithidia fasciculataand Trypanosoma cruzi.The constituents were initially added individually at different concentrations to C. fasciculatacultures to estimate the IC50/24h. Concentrations in a triple combination were about 2 times and 16.5 times lower against C. fasciculata and T. cruzi, respectively, as compared to isolated compounds. Incubation of C. fasciculatawith the trypanocydal agent benznidazole did not affect parasite growth at concentrations up to 500 µg/ml, but the IC50 of this drug against T. cruziwas 15.8 µg/ml, a value about 2-5 times higher than that of constituents in the triple combination. Analysis of treated C. fasciculata by scanning electron microscopy showed rounding of the cell body. Our data show that combination of essential oil constituents resulted in increased inhibitory activity on growth of both non-pathogenic and pathogenic trypanosomatid species and indicate that the non-patogenic C. fasciculata may represent a resistant model for drug screening in trypanosomatids.
| Main Authors: | , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Sociedade Brasileira de Farmacognosia
2013
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2013000500762 |
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| Summary: | We analyzed the effect of the combination of citral, eugenol and thymol, respectively the main constituents of essential oils of Cympobogon citratus (DC) Stapf, Poaceae (lemon grass), Syzygium aromaticum(L.) Merr. & L.M. Perry, Myrtaceae (clove) and Thymus vulgarisL., Lamiaceae (thyme), on the proliferation of the trypanosomatids Crithidia fasciculataand Trypanosoma cruzi.The constituents were initially added individually at different concentrations to C. fasciculatacultures to estimate the IC50/24h. Concentrations in a triple combination were about 2 times and 16.5 times lower against C. fasciculata and T. cruzi, respectively, as compared to isolated compounds. Incubation of C. fasciculatawith the trypanocydal agent benznidazole did not affect parasite growth at concentrations up to 500 µg/ml, but the IC50 of this drug against T. cruziwas 15.8 µg/ml, a value about 2-5 times higher than that of constituents in the triple combination. Analysis of treated C. fasciculata by scanning electron microscopy showed rounding of the cell body. Our data show that combination of essential oil constituents resulted in increased inhibitory activity on growth of both non-pathogenic and pathogenic trypanosomatid species and indicate that the non-patogenic C. fasciculata may represent a resistant model for drug screening in trypanosomatids. |
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