Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops

Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops

Abstract: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.

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Bibliographic Details
Main Authors: Branco,Érika, Silva,Klena Sarges Marruaz, Assis,Maria de Fátima, Oliveira,Edivaldo Herculano Corrêa de, Lima,Ana Rita de, Aquino,Ana Cleide Guimbal de, Cabral,Rosa, Miglino,Maria Angelica
Format: Digital revista
Language:English
Published: Colégio Brasileiro de Patologia Animal - CBPA 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000200119
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Summary:Abstract: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.

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