EVALUATION OF ENZYMES FOR THE HYDROLYSIS OF WASTE (LEAVES AND TOP CANE) FROM THE HARVEST OF SUGAR CANE
The enzymatic hydrolysis of waste (leaves and top cane) from the harvest of sugar cane was evaluated. The substrates were prepared by applying different delignification treatments using enzymes, organic solvents, sodium hydroxide, and sodium chlorite. Wastes with no treatment were used as control. Five mixtures of enzymes were prepared by using the enzymatic activities of the commercial enzymes, and were evaluated in the different substrates. A criterion for evaluating the efficiency of enzymes from progress curves, percentage saccharification and saccharification index, called "global index of hydrolysis" was introduced. The results shows that the hydrolysis with E5 enzymatic preparation with the following enzyme activities, hemicelullases: 52.75 global units of /ml, total cellulase: 27.53 FPU / ml, endoglucanase: 1782.1 CMC / ml, exoglucanase: 0.377 UI / ml, β-glucosidase: 550 pUPG U / ml, xylanase: 28.23 UI / ml, galactosidasa: 7.1 UI / ml, manase: 2.76 UI / ml, and ranmanasa: 14.63 UI / ml, and the S3 substrate with 80.53% of cellulose content, 13.35% of hemicellulose, 1,36 % of lignin, and Kappa Number of 5.85 has the highest overall index of hydrolysis,117.16. The study concludes that this enzyme preparation, E5, has greatest potential for the hydrolysis the harvest waste of sugarcane used in this study.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Universidad Nacional de Colombia
2011
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| Online Access: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0012-73532011000500021 |
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| Summary: | The enzymatic hydrolysis of waste (leaves and top cane) from the harvest of sugar cane was evaluated. The substrates were prepared by applying different delignification treatments using enzymes, organic solvents, sodium hydroxide, and sodium chlorite. Wastes with no treatment were used as control. Five mixtures of enzymes were prepared by using the enzymatic activities of the commercial enzymes, and were evaluated in the different substrates. A criterion for evaluating the efficiency of enzymes from progress curves, percentage saccharification and saccharification index, called "global index of hydrolysis" was introduced. The results shows that the hydrolysis with E5 enzymatic preparation with the following enzyme activities, hemicelullases: 52.75 global units of /ml, total cellulase: 27.53 FPU / ml, endoglucanase: 1782.1 CMC / ml, exoglucanase: 0.377 UI / ml, β-glucosidase: 550 pUPG U / ml, xylanase: 28.23 UI / ml, galactosidasa: 7.1 UI / ml, manase: 2.76 UI / ml, and ranmanasa: 14.63 UI / ml, and the S3 substrate with 80.53% of cellulose content, 13.35% of hemicellulose, 1,36 % of lignin, and Kappa Number of 5.85 has the highest overall index of hydrolysis,117.16. The study concludes that this enzyme preparation, E5, has greatest potential for the hydrolysis the harvest waste of sugarcane used in this study. |
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