The in vitro effect of leukocyte- and platelet-rich fibrin (L-PRF) and cross-linked hyaluronic acid on fibroblast viability and proliferation
INTRODUCTION: Leukocyte- and platelet-rich fibrin (L-PRF), an autologous derived platelet and leukocyte concentrate, was first introduced by Choukroun et al. in 2001 and is currently used for several oral and maxillofacial procedures. Hyaluronic acid (HA) is an anionic glycosaminoglycan that shows a wide range of physiological actions, acting as a cellular scaffold, stimulating cell adhesion and migration, as well as having anti-inflammatory effects. AIMS AND OBJECTIVES: To investigate the effect of cross-linked HA and L-PRF on fibroblast viability and proliferation. METHODS: An in vitro laboratory study was conducted at The University of the Western Cape. L-PRF was prepared from a single healthy volunteer and cross-linked HA was obtained from a commercially available product. Analysis was carried out on 3T3 cells using MTT assay RESULTS: Both L-PRF and cross-linked HA are associated with cell viability and increased cell proliferation. L-PRF had it strongest proliferative effect after 24 hours whereas HA had it strongest effect after eight days (p < 0.05). The combination of HA and L-PRF was not significantly better than the control (p > 0.05). CONCLUSIONS: L-PRF prepared in a specific manner as outlined, as well as commercially available cross-linked HA, is compatible with cell growth and proliferation.
| Main Authors: | , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
The South African Dental Association (SADA)
2018
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| Online Access: | http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0011-85162018000600004 |
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| Summary: | INTRODUCTION: Leukocyte- and platelet-rich fibrin (L-PRF), an autologous derived platelet and leukocyte concentrate, was first introduced by Choukroun et al. in 2001 and is currently used for several oral and maxillofacial procedures. Hyaluronic acid (HA) is an anionic glycosaminoglycan that shows a wide range of physiological actions, acting as a cellular scaffold, stimulating cell adhesion and migration, as well as having anti-inflammatory effects. AIMS AND OBJECTIVES: To investigate the effect of cross-linked HA and L-PRF on fibroblast viability and proliferation. METHODS: An in vitro laboratory study was conducted at The University of the Western Cape. L-PRF was prepared from a single healthy volunteer and cross-linked HA was obtained from a commercially available product. Analysis was carried out on 3T3 cells using MTT assay RESULTS: Both L-PRF and cross-linked HA are associated with cell viability and increased cell proliferation. L-PRF had it strongest proliferative effect after 24 hours whereas HA had it strongest effect after eight days (p < 0.05). The combination of HA and L-PRF was not significantly better than the control (p > 0.05). CONCLUSIONS: L-PRF prepared in a specific manner as outlined, as well as commercially available cross-linked HA, is compatible with cell growth and proliferation. |
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