Aislamiento y selección de hongos endófitos nativos con potencial antagónico a nematodos fitoparásitos en plantaciones de banano en Valverde y Montecristi.
In the Dominican Republic, banana production (Musa AAA) has become an important agricultural activity for the generation of foreign exchange and jobs, with a significant increase since 1990s. This crop, like the other musaceae, is affected by nematodes phytoparasites that destroy the root system of plants. The objective of this research was to isolate and identify native fungi with antagonistic potential to phytoparasitic nematodes in banana plantations. 20 banana farms were sampled, 10 in Valverde province and 10 in Montecristi. The samples were processed in the laboratory of the Mata Larga Experimental Station of the IDIAF, San Francisco de Macorís. Apparently healthy roots were selected, washed and cut into 3 to 4 cm long pieces and disinfected in 2% chlorine for three minutes, passed three times through sterile distilled water. Then they were seeded in Petri dishes with PDA 10%. Four plates were used per sample, in each plate five pieces of roots were placed. Then they were incubated for 5 to 7 days at 28 ± 2 ºC, and the developed colonies were identified based on their morphological characteristics. In the samples from Valverde, 214 colonies of endophytic fungi were isolated, 13 correspond to the Trichoderma genus and 201 to Fusarium. In those from Montecristi, 209 colonies were isolated, of which 53 correspond to the genus Trichoderma and 156 to Fusarium. Of the genus Trichoderma, 19 strains were conserved and of the genus Fusarium 18 non-pathogenic strains.
| Main Authors: | , , , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Sociedad Dominicana de Investigadores Agropecuarios y Forestales
2021
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| Online Access: | https://www.sodiaf.org.do/apf/index.php/apf/article/view/126 |
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| Summary: | In the Dominican Republic, banana production (Musa AAA) has become an important agricultural activity for the generation of foreign exchange and jobs, with a significant increase since 1990s. This crop, like the other musaceae, is affected by nematodes phytoparasites that destroy the root system of plants. The objective of this research was to isolate and identify native fungi with antagonistic potential to phytoparasitic nematodes in banana plantations. 20 banana farms were sampled, 10 in Valverde province and 10 in Montecristi. The samples were processed in the laboratory of the Mata Larga Experimental Station of the IDIAF, San Francisco de Macorís. Apparently healthy roots were selected, washed and cut into 3 to 4 cm long pieces and disinfected in 2% chlorine for three minutes, passed three times through sterile distilled water. Then they were seeded in Petri dishes with PDA 10%. Four plates were used per sample, in each plate five pieces of roots were placed. Then they were incubated for 5 to 7 days at 28 ± 2 ºC, and the developed colonies were identified based on their morphological characteristics. In the samples from Valverde, 214 colonies of endophytic fungi were isolated, 13 correspond to the Trichoderma genus and 201 to Fusarium. In those from Montecristi, 209 colonies were isolated, of which 53 correspond to the genus Trichoderma and 156 to Fusarium. Of the genus Trichoderma, 19 strains were conserved and of the genus Fusarium 18 non-pathogenic strains. |
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