SEARCH FOR 1RS RYE (Secale cereale) TRANSLOCATIONS IN WHEAT (Triticum durum) VARIETIES FROM THE BAJÍO AREA, MEXICO.
The 1AL.1RS and 1BL.1RS translocations are widely used in wheat (Triticum durum) breeding programs in several countries and allowed varieties development with improved agronomic traits, such as resistance to abiotic and biotic stress. Our objective in this study was to identify and select genotypes based on the presence of 1AL.1RS and 1BL.1RS translocations on the main used wheat varieties from the Mexican Bajío. The hypothesis was that the presence of 1AL.1RS and 1BL.1RS translocations would be different among the studied varieties. The commercial and extensively cultivated in the Bajío wheat varieties were Cortázar, Maya, Bárcenas, Salamanca and Santa Ana, the internal experimental controls were the drought tolerant Nana variety and the susceptible Júpare, the TAM 200 variety as external control, in which the presence of the 1AL.1RS translocation was included. The genomic DNA of 20 plants of each variety was extracted and the presence or absence of the 1AL.1RS and 1BL.1RS translocations was evaluated by the PCR amplification of five markers. The results of the diagnosis were analyzed in a completely randomized design with 20 repetitions using the Kruskal-Wallis test. None of the commercial varieties or internal experimental controls showed 1AL.1RS or 1BL.1RS translocations. This indicates that in the local wheat breeding programs have not used translocations to identify and select outstanding genotypes. However, the RYE-NOR and RIS markers indicated the presence of not reported translocations, most likely due to introgression with local rye (Secale cereale) sources. None of the evaluated commercial varieties exhibit 1AL.1RS or 1BL.1RS translocations, but the RYE-NOR and RIS markers indicated the presence of previously unreported translocations.
| Main Authors: | , , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Colegio de Postgraduados
2019
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| Online Access: | https://www.agrociencia-colpos.org/index.php/agrociencia/article/view/1783 |
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| Summary: | The 1AL.1RS and 1BL.1RS translocations are widely used in wheat (Triticum durum) breeding programs in several countries and allowed varieties development with
improved agronomic traits, such as resistance to abiotic and biotic stress. Our objective in this study was to identify and select genotypes based on the presence of 1AL.1RS and 1BL.1RS
translocations on the main used wheat varieties from the Mexican Bajío. The hypothesis was that the presence of 1AL.1RS and 1BL.1RS translocations would be different among the studied
varieties. The commercial and extensively cultivated in the Bajío wheat varieties were Cortázar, Maya, Bárcenas, Salamanca and Santa Ana, the internal experimental controls were the drought
tolerant Nana variety and the susceptible Júpare, the TAM 200 variety as external control, in which the presence of the 1AL.1RS translocation was included. The genomic DNA of 20 plants of
each variety was extracted and the presence or absence of the 1AL.1RS and 1BL.1RS translocations was evaluated by the PCR amplification of five markers. The results of the diagnosis were
analyzed in a completely randomized design with 20 repetitions using the Kruskal-Wallis test. None of the commercial varieties or internal experimental controls showed 1AL.1RS or 1BL.1RS
translocations. This indicates that in the local wheat breeding programs have not used translocations to identify and select outstanding genotypes. However, the RYE-NOR and RIS markers
indicated the presence of not reported translocations, most likely due to introgression with local rye (Secale cereale) sources. None of the evaluated commercial varieties exhibit 1AL.1RS or
1BL.1RS translocations, but the RYE-NOR and RIS markers indicated the presence of previously unreported translocations. |
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