Essential oils and benzylaminopurine (BAP) for multiplication in vitro of Dimorphandra mollis
Micropropagation is an alternative for genetic conservation of Cerrado endemic species, such as Dimorphandra mollis (fava-d´anta), making possible maximizing propagation in high quality. To solve the limiting factors of in vitro cultivation of D. mollis, the present study aimed to evaluate the efficiency of essential oils as a sanitizer and to test concentrations of benzylaminopurine growth regulator (BAP) in the in vitro multiplication phase. Seeds collected from a natural population were scarified and disinfected with sodium hypochlorite and essential oils of Lippia origanoides, Ocimum gratissimum, L. rotundifolia and Cymbopogon citratus. The seeds were inoculated in MS culture medium. In vitro multiplication was evaluated using four concentrations of BAP (0.5; 1.0; 1.5 and 2.0 mg L-1). Essential oil of C. citratus is the most suitable for disinfestation of fava d´anta seeds, in the stage of establishment in vitro. Phytoregulators used in the present study did not provide satisfactory results to define an in vitro multiplication protocol for the species.
| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | por |
| Published: |
Embrapa Florestas
2021
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| Online Access: | https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1926 |
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| Summary: | Micropropagation is an alternative for genetic conservation of Cerrado endemic species, such as Dimorphandra mollis (fava-d´anta), making possible maximizing propagation in high quality. To solve the limiting factors of in vitro cultivation of D. mollis, the present study aimed to evaluate the efficiency of essential oils as a sanitizer and to test concentrations of benzylaminopurine growth regulator (BAP) in the in vitro multiplication phase. Seeds collected from a natural population were scarified and disinfected with sodium hypochlorite and essential oils of Lippia origanoides, Ocimum gratissimum, L. rotundifolia and Cymbopogon citratus. The seeds were inoculated in MS culture medium. In vitro multiplication was evaluated using four concentrations of BAP (0.5; 1.0; 1.5 and 2.0 mg L-1). Essential oil of C. citratus is the most suitable for disinfestation of fava d´anta seeds, in the stage of establishment in vitro. Phytoregulators used in the present study did not provide satisfactory results to define an in vitro multiplication protocol for the species. |
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