Immune response in rabbits to two sizes of cysts of sarcocystis aucheniae
The aim of this study was to evaluate the immune response of small and large macrocysts of Sarcocystis auchenie using three protocols of inoculation. Alpaca meat samples with S. aucheniae were obtained from the slaughterhouse of Huancavelica, Peru. Cysts were collected and classified by size in large (>5 mm) and small (1-3 mm), obtaining 3.23 and 2.45 mg/ml respectively of antigenic protein by the Biuret method. Fifteen rabbits were immunized with 0.05 mg/kg of antigenic protein derived from large and small macrocysts. Besides, three protocols of inoculation were considered (n = 5 rabitts). Protocol I and II were of 1 and 3 month length where proteins were inactivated with formalin, and protocol III of 2 months length using heat-inactivated antigen protein. Specific immune response Ag-Ab was detected by double immunodiffusion in agar gel in 100% of rabbits using protocols I and II and 60% with protocol III in large macrocysts while 100, 60 and 40% response in protocols I, II and III in small macrocysts respectively. In conclusion, immune responses of 86.6 and 66.6% were obtained when using antigenic proteins from large and small cysts, respectively; however, no association was observed between protocols and Ag-Ab reaction when using Fisher’s analysis.
| Main Authors: | , , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
2012
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| Online Access: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/902 |
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| Summary: | The aim of this study was to evaluate the immune response of small and large macrocysts of Sarcocystis auchenie using three protocols of inoculation. Alpaca meat samples with S. aucheniae were obtained from the slaughterhouse of Huancavelica, Peru. Cysts were collected and classified by size in large (>5 mm) and small (1-3 mm), obtaining 3.23 and 2.45 mg/ml respectively of antigenic protein by the Biuret method. Fifteen rabbits were immunized with 0.05 mg/kg of antigenic protein derived from large and small macrocysts. Besides, three protocols of inoculation were considered (n = 5 rabitts). Protocol I and II were of 1 and 3 month length where proteins were inactivated with formalin, and protocol III of 2 months length using heat-inactivated antigen protein. Specific immune response Ag-Ab was detected by double immunodiffusion in agar gel in 100% of rabbits using protocols I and II and 60% with protocol III in large macrocysts while 100, 60 and 40% response in protocols I, II and III in small macrocysts respectively. In conclusion, immune responses of 86.6 and 66.6% were obtained when using antigenic proteins from large and small cysts, respectively; however, no association was observed between protocols and Ag-Ab reaction when using Fisher’s analysis. |
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