Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections.
| Main Authors: | , , , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | eng |
| Published: |
Elsevier
2021-07
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| Subjects: | Bacterias Acidolácticas, Lactobacillus, Lactobacillus casei, Genética, Lactic Acid Bacteria, Genetics, Lacticaseibacillus, CRISPR systems, |
| Online Access: | http://hdl.handle.net/20.500.12123/9014 https://www.sciencedirect.com/science/article/abs/pii/S0958694621000558 https://doi.org/10.1016/j.idairyj.2021.105027 |
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| Summary: | A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections. |
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