Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators

The Paraná River basin is one of the most important in South America and is affected by human activities that take place on its margins. In particular, the De la Cruz stream flows through an industrial pole and the Arrecifes River goes mainly through agricultural fields. The aim of this study was to evaluate the water quality of the De la Cruz stream (S1) and the Arrecifes River (S2) by means of physicochemical parameters, including metals and pesticides concentrations. Since amphibians are good indicators of environmental quality, bioassays with Rhinella arenarum were carried on. For lethal and sublethal parameters, embryos and larvae were exposed to a dilution gradient of water samples and AMPHITOX Solution (AS) as negative control for 504 h. For the determination of oxidative stress biomarkers (Catalase -CAT-, Glutathione S-Transferase -GST-, Reduced Glutathione -GSH-, and lipid peroxidation -TBARS-), embryos and larvae were exposed to undiluted water samples and AS. For the determination of micronuclei, larvae at hind limb bud stage (S.28) were exposed to undiluted water samples, simultaneously with negative and positive controls (AS and cyclophosphamide 40 mg/L, respectively). Dissolved oxygen was low in both sites and the copper levels exceeded the Argentine limit for the protection of aquatic life. In embryos exposure, water sample from S1 caused lethal effects (504h-LC50 = 49 (28–71.6)%), increased TBARS levels, and GST and CAT activities. In larvae exposure, water sample from this site decreased CAT activity, while the water sample from S2 caused important lethal effects (504h-LC50 = 98.72 (60.60–302.52)%), low GSH levels and increased GST activity. Water samples from both sites induced higher micronuclei frequency than the negative control. This study alerts about the degradation of water quality of the studied sites including lethal and sublethal effects in R. arenarum that can jeopardize the native populations of this species.

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Bibliographic Details
Main Authors: Peluso, Julieta, Aronzon, Carolina Mariel, Ríos de Molina, María del Carmen, Rojas, Dante Emanuel, Cristos, Diego Sebastian, Pérez Coll, Cristina Silvia
Format: info:ar-repo/semantics/artículo biblioteca
Language:eng
Published: Elsevier 2020-03-20
Subjects:Amphibians, Genotoxicity, Oxidative Stress, Water Quality, Anfibios, Genotoxicidad, Estrés Oxidativo, Calidad del Agua, Río Paraná, Argentina,
Online Access:http://hdl.handle.net/20.500.12123/7165
https://www.sciencedirect.com/science/article/pii/S0269749119340886
https://doi.org/10.1016/j.envpol.2020.114434
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record_format koha
institution INTA AR
collection DSpace
country Argentina
countrycode AR
component Bibliográfico
access En linea
databasecode dig-inta-ar
tag biblioteca
region America del Sur
libraryname Biblioteca Central del INTA Argentina
language eng
topic Amphibians
Genotoxicity
Oxidative Stress
Water Quality
Anfibios
Genotoxicidad
Estrés Oxidativo
Calidad del Agua
Río Paraná, Argentina
Amphibians
Genotoxicity
Oxidative Stress
Water Quality
Anfibios
Genotoxicidad
Estrés Oxidativo
Calidad del Agua
Río Paraná, Argentina
spellingShingle Amphibians
Genotoxicity
Oxidative Stress
Water Quality
Anfibios
Genotoxicidad
Estrés Oxidativo
Calidad del Agua
Río Paraná, Argentina
Amphibians
Genotoxicity
Oxidative Stress
Water Quality
Anfibios
Genotoxicidad
Estrés Oxidativo
Calidad del Agua
Río Paraná, Argentina
Peluso, Julieta
Aronzon, Carolina Mariel
Ríos de Molina, María del Carmen
Rojas, Dante Emanuel
Cristos, Diego Sebastian
Pérez Coll, Cristina Silvia
Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators
description The Paraná River basin is one of the most important in South America and is affected by human activities that take place on its margins. In particular, the De la Cruz stream flows through an industrial pole and the Arrecifes River goes mainly through agricultural fields. The aim of this study was to evaluate the water quality of the De la Cruz stream (S1) and the Arrecifes River (S2) by means of physicochemical parameters, including metals and pesticides concentrations. Since amphibians are good indicators of environmental quality, bioassays with Rhinella arenarum were carried on. For lethal and sublethal parameters, embryos and larvae were exposed to a dilution gradient of water samples and AMPHITOX Solution (AS) as negative control for 504 h. For the determination of oxidative stress biomarkers (Catalase -CAT-, Glutathione S-Transferase -GST-, Reduced Glutathione -GSH-, and lipid peroxidation -TBARS-), embryos and larvae were exposed to undiluted water samples and AS. For the determination of micronuclei, larvae at hind limb bud stage (S.28) were exposed to undiluted water samples, simultaneously with negative and positive controls (AS and cyclophosphamide 40 mg/L, respectively). Dissolved oxygen was low in both sites and the copper levels exceeded the Argentine limit for the protection of aquatic life. In embryos exposure, water sample from S1 caused lethal effects (504h-LC50 = 49 (28–71.6)%), increased TBARS levels, and GST and CAT activities. In larvae exposure, water sample from this site decreased CAT activity, while the water sample from S2 caused important lethal effects (504h-LC50 = 98.72 (60.60–302.52)%), low GSH levels and increased GST activity. Water samples from both sites induced higher micronuclei frequency than the negative control. This study alerts about the degradation of water quality of the studied sites including lethal and sublethal effects in R. arenarum that can jeopardize the native populations of this species.
format info:ar-repo/semantics/artículo
topic_facet Amphibians
Genotoxicity
Oxidative Stress
Water Quality
Anfibios
Genotoxicidad
Estrés Oxidativo
Calidad del Agua
Río Paraná, Argentina
author Peluso, Julieta
Aronzon, Carolina Mariel
Ríos de Molina, María del Carmen
Rojas, Dante Emanuel
Cristos, Diego Sebastian
Pérez Coll, Cristina Silvia
author_facet Peluso, Julieta
Aronzon, Carolina Mariel
Ríos de Molina, María del Carmen
Rojas, Dante Emanuel
Cristos, Diego Sebastian
Pérez Coll, Cristina Silvia
author_sort Peluso, Julieta
title Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators
title_short Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators
title_full Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators
title_fullStr Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators
title_full_unstemmed Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators
title_sort integrated analysis of the quality of water bodies from the lower paraná river basin with different productive uses by physicochemical and biological indicators
publisher Elsevier
publishDate 2020-03-20
url http://hdl.handle.net/20.500.12123/7165
https://www.sciencedirect.com/science/article/pii/S0269749119340886
https://doi.org/10.1016/j.envpol.2020.114434
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spelling oai:localhost:20.500.12123-71652020-04-28T19:58:32Z Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators Peluso, Julieta Aronzon, Carolina Mariel Ríos de Molina, María del Carmen Rojas, Dante Emanuel Cristos, Diego Sebastian Pérez Coll, Cristina Silvia Amphibians Genotoxicity Oxidative Stress Water Quality Anfibios Genotoxicidad Estrés Oxidativo Calidad del Agua Río Paraná, Argentina The Paraná River basin is one of the most important in South America and is affected by human activities that take place on its margins. In particular, the De la Cruz stream flows through an industrial pole and the Arrecifes River goes mainly through agricultural fields. The aim of this study was to evaluate the water quality of the De la Cruz stream (S1) and the Arrecifes River (S2) by means of physicochemical parameters, including metals and pesticides concentrations. Since amphibians are good indicators of environmental quality, bioassays with Rhinella arenarum were carried on. For lethal and sublethal parameters, embryos and larvae were exposed to a dilution gradient of water samples and AMPHITOX Solution (AS) as negative control for 504 h. For the determination of oxidative stress biomarkers (Catalase -CAT-, Glutathione S-Transferase -GST-, Reduced Glutathione -GSH-, and lipid peroxidation -TBARS-), embryos and larvae were exposed to undiluted water samples and AS. For the determination of micronuclei, larvae at hind limb bud stage (S.28) were exposed to undiluted water samples, simultaneously with negative and positive controls (AS and cyclophosphamide 40 mg/L, respectively). Dissolved oxygen was low in both sites and the copper levels exceeded the Argentine limit for the protection of aquatic life. In embryos exposure, water sample from S1 caused lethal effects (504h-LC50 = 49 (28–71.6)%), increased TBARS levels, and GST and CAT activities. In larvae exposure, water sample from this site decreased CAT activity, while the water sample from S2 caused important lethal effects (504h-LC50 = 98.72 (60.60–302.52)%), low GSH levels and increased GST activity. Water samples from both sites induced higher micronuclei frequency than the negative control. This study alerts about the degradation of water quality of the studied sites including lethal and sublethal effects in R. arenarum that can jeopardize the native populations of this species. Fil: Peluso, Julieta. Universidad Nacional de San Martín (UNSAM). Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Aronzon, Carolina Mariel. Universidad Nacional de San Martín (UNSAM). Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Fil: Ríos de Molina, María del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Rojas, Dante Emanuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica; Argentina. Fil: Cristos, Diego Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica; Argentina. Fil: Pérez Coll, Cristina Silvia. Universidad Nacional de San Martín (UNSAM). Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. 2020-04-28T19:39:03Z 2020-04-28T19:39:03Z 2020-03-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7165 https://www.sciencedirect.com/science/article/pii/S0269749119340886 0269-7491 https://doi.org/10.1016/j.envpol.2020.114434 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Environmental Pollution (263) : Part B. 114434 (2020)