Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species = Desarrollo y caracterización de marcadores moleculares SSR para Trichloris crinita usando secuencias de gramíneas filogenéticamente cercanas
Trichloris crinita is among the most important native forage grasses in arid regions of America. Despite its importance, molecular resources and sequence data are extremely scarce in this species. In the present study, SSR markers were developed using available DNA sequences from grass taxa phylogenetically-related to Trichloris (Eleusine coracana, Cynodon dactylon and ‘Cynodon dactylon x Cynodon transvaalensis’). Marker transferability was evaluated in a panel of eight T. crinita accessions and five closely-related species. Of the 105 SSR primer pairs evaluated, 16 amplified products of expected size in T. crinita, whereas transferability to other grass species ranged from 12 (in Chloris castilloniana) to 28 SSRs (in Eleusine coracana). Six of the 16 SSR markers successfully transferred to T. crinita (37.5%) were polymorphic, and were further used to assess genetic diversity in eight T. crinita accessions. The analysis revealed a total of 23 SSR alleles (3.83 alleles/locus), allowing the discrimination of all T. crinita accessions, with pair-wise genetic similarities ranging from 0.35 to 0.81 (Jaccard coefficient). Mean (and range) values for observed (Ho) and expected heterozygosity (He) were 0.53 (0.0-1.0) and 0.63 (0.48-0.79), respectively.
| Main Authors: | , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | eng |
| Published: |
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
2018
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| Subjects: | Gramíneas Forrajeras, Marcadores Genéticos, Variación Genética, Microsatélites, Microsatellites, Genetic Variation, Genetic Markers, Feed Grasses, Chloridoideae, Trichloris Crinita, Marcadores Moleculares SSR, Transferibilidad, Marker Transferability, |
| Online Access: | http://hdl.handle.net/20.500.12123/2996 http://revista.fca.uncu.edu.ar/index.php?option=com_content&view=article&id=561:2018-05-29-17-58-40&catid=27:2018-05-29-17-20-51&Itemid=35 |
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| Summary: | Trichloris crinita is among the most important native forage grasses in arid regions
of America. Despite its importance, molecular resources and sequence data are
extremely scarce in this species. In the present study, SSR markers were developed
using available DNA sequences from grass taxa phylogenetically-related to Trichloris
(Eleusine coracana, Cynodon dactylon and ‘Cynodon dactylon x Cynodon transvaalensis’).
Marker transferability was evaluated in a panel of eight T. crinita accessions and five
closely-related species. Of the 105 SSR primer pairs evaluated, 16 amplified products
of expected size in T. crinita, whereas transferability to other grass species ranged from
12 (in Chloris castilloniana) to 28 SSRs (in Eleusine coracana). Six of the 16 SSR markers
successfully transferred to T. crinita (37.5%) were polymorphic, and were further used
to assess genetic diversity in eight T. crinita accessions. The analysis revealed a total of
23 SSR alleles (3.83 alleles/locus), allowing the discrimination of all T. crinita accessions,
with pair-wise genetic similarities ranging from 0.35 to 0.81 (Jaccard coefficient). Mean
(and range) values for observed (Ho) and expected heterozygosity (He) were 0.53
(0.0-1.0) and 0.63 (0.48-0.79), respectively. |
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