Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration.
| Main Authors: | , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | eng |
| Published: |
2011-12-12
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| Subjects: | RNA, Metabolismo, Virus de las Plantas, Nicotiana Tabacum, Tabaco, Cromatografía Gas-líquido, Espectrometría de Masas, Mass Spectrometry, Gas Liquid Chromatography, Tobacco, Plant Viruses, Virus del Mosaico del Tabaco, miRNA, |
| Online Access: | http://hdl.handle.net/20.500.12123/2168 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236191/ |
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| Summary: | Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites
and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in
signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic
approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of
metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes
in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the
action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were
hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where
no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a
massive increase in the density of correlation networks after infection indicating a complex reprogramming of the
regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the
involvement of a systemic signaling on early miRs alteration. |
|---|