Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle
A simple IgG-specific ELISA for Leptospira spp. was compared with the microscopic agglutination test (MAT) to detect IgG antibody responses to a commercial vaccine in cattle. We used an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serovar copenhageni M 20. After initial vaccination, specific antibodies against Leptospira spp. were detected in 90% of the animals by IgG-ELISA and 60% by MAT, while after booster, antibodies were detected in 100% and 90% of the animals by IgG-ELISA and MAT, respectively. Both serological MAT and ELISA tests revealed interferences of vaccine antibodies. Disease diagnosis with ELISA and MAT methods should be made two and a half months and four months, respectively, after vaccination to avoid interference of vaccine antibodies. On the other hand, our results suggest that IgG-ELISA may be a useful method to assess the development of IgG antibodies induced by Leptospira vaccine.
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | eng |
| Published: |
Elsevier
2024
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| Subjects: | Cattle, ELISA, Vaccines, Leptospirosis, Antibodies, Immunodiagnosis, Agglutination Tests, Ganado Bovino, Vacuna, Anticuerpo, Inmunodiagnóstico, Reacciones de Aglutinación, |
| Online Access: | http://hdl.handle.net/20.500.12123/18583 https://www.sciencedirect.com/science/article/abs/pii/S0378113524001913 https://doi.org/10.1016/j.vetmic.2024.110169 |
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| Summary: | A simple IgG-specific ELISA for Leptospira spp. was compared with the microscopic agglutination test (MAT) to detect IgG antibody responses to a commercial vaccine in cattle. We used an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serovar copenhageni M 20. After initial vaccination, specific antibodies against Leptospira spp. were detected in 90% of the animals by IgG-ELISA and 60% by MAT, while after booster, antibodies were detected in 100% and 90% of the animals by IgG-ELISA and MAT, respectively. Both serological MAT and ELISA tests revealed interferences of vaccine antibodies. Disease diagnosis with ELISA and MAT methods should be made two and a half months and four months, respectively, after vaccination to avoid interference of vaccine antibodies. On the other hand, our results suggest that IgG-ELISA may be a useful method to assess the development of IgG antibodies induced by Leptospira vaccine. |
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