Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids. Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci. Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.

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Bibliographic Details
Main Authors: Pomponio, Maria Florencia, Acuña, Cintia Vanesa, Petreath, Vivien, Lopez Lauenstein, Diego, Marcucci Poltri, Susana Noemi, Torales, Susana Leonor
Format: info:ar-repo/semantics/artículo biblioteca
Language:eng
Published: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) 2015
Subjects:Prosopis alba, Marcadores Genéticos, Prosopis, Variación Genética, Microsatélites, Genetic Markers, Genetic Variation, Microsatellites, Marcadores SSR, SSR Markers,
Online Access:http://hdl.handle.net/20.500.12123/11356
https://revistas.inia.es/index.php/fs/article/view/7188
https://doi.org/10.5424/fs/2015242-07188
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Summary:Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids. Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci. Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.