Contrasting rates of protein and morphological evolution in cyst nematode species
The genetic differentiation of cyst nematode species that are morphologically nearly indistinguishable was investigated by means of two-dimensional gel electrophoresis (2-DGE) followed by a sensitive silver stain. 2-DGE of total protein extracts from young females revealed an average of 245 polypeptides. The sibling species Globodera rostochiensis and G. pallida were differentiated by 70% of their polypeptides. The closely related species Heterodera glycines and H. schachtii were discriminated by 59% of their polypeptides. These large differences suggest that these nematode species have accumulated protein differences during a period of millions of years without distinct changes in morphology. These observations emphasize the importance of biochemical techniques in plant nematology for species identification and nematode systematics.
| Main Authors: | , |
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | Life Science, |
| Online Access: | https://research.wur.nl/en/publications/contrasting-rates-of-protein-and-morphological-evolution-in-cyst- |
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| Summary: | The genetic differentiation of cyst nematode species that are morphologically nearly indistinguishable was investigated by means of two-dimensional gel electrophoresis (2-DGE) followed by a sensitive silver stain. 2-DGE of total protein extracts from young females revealed an average of 245 polypeptides. The sibling species Globodera rostochiensis and G. pallida were differentiated by 70% of their polypeptides. The closely related species Heterodera glycines and H. schachtii were discriminated by 59% of their polypeptides. These large differences suggest that these nematode species have accumulated protein differences during a period of millions of years without distinct changes in morphology. These observations emphasize the importance of biochemical techniques in plant nematology for species identification and nematode systematics. |
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