Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus

Plant viruses and their virus-like particles (VLPs) have a lot of advantages for biotechnological applications including complete safety for humans. Alternanthera mosaic virus (AltMV) is a potentially promising object for design of novel materials. The 3D structures of AltMV virions and its VLPs were obtained by single particle EM at ~13Å resolution. The comparison of the reconstructions and a trypsin treatment revealed that AltMV CPs possesses a different fold in the presence (virions) and absence of viral RNA (VLPs). For the first time, the structure of morphologically similar virions and virus-like particles based on the coat protein of a helical filamentous plant virus is shown to be different. Despite this, both AltMV virions and VLPs are stable in a wide range of conditions. To provide a large amount of AltMV for biotechnology usage the isolation procedure was modified.

Bibliographic Details

Main Authors:	Donchenko, Ekaterina K., Pechnikova, Evgeniya V., Mishyna, Maryia Yu, Manukhova, Tatiana I., Sokolova, Olga S., Nikitin, Nikolai A., Atabekov, Joseph G., Karpova, Olga V.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	Life Science,
Online Access:	https://research.wur.nl/en/publications/structure-and-properties-of-virions-and-virus-like-particles-deri
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