A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range

Large-scale monitoring of foot-and-mouth disease (FMD) in livestock is imperative in an FMD control program. Detection of antibodies against non-structural proteins (NSP) of FMD virus (FMDV) is one of the best tools to estimate the prevalence of past infection; availability of such a well-validated test is therefore essential. Using a FMDV 3B protein-specific monoclonal antibody, we have developed a new NSP antibody blocking ELISA (10H9 bELISA) and validated it on large panels of sera from different susceptible species. The diagnostic sensitivity of the ELISA was 95% with a specificity of 98%, similar to the values found using a commercial kit (PrioCHECK FMD NS test). The 10H9 bELISA can be used in a broad range of FMD susceptible species making it a very useful tool in monitoring the foot-and-mouth disease control programs by detection of virus circulation in the vaccinated populations. Key points: • A new ELISA for detection of foot and mouth disease (FMD) antibodies. • Diagnostic sensitivity of 95% and specificity of 98%. • Tested with panels of validated sera from broad host range.

Bibliographic Details

Main Authors:	Hosamani, Madhusudan, Gopinath, Shreya, Sreenivasa, B.P., Behera, Subhasmita, Basagoudanavar, Suresh H., Boora, Ashok, Bora, Durlav P., Deka, Pankaj, Bhanuprakash, Veerakyathappa, Singh, Raj Kumar, Sanyal, Aniket, Weerdmeester, Klaas, Dekker, Aldo
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	Blocking ELISA, Foot-and-mouth disease, Monoclonal antibody, Non-structural protein antibodies,
Online Access:	https://research.wur.nl/en/publications/a-new-blocking-elisa-for-detection-of-foot-and-mouth-disease-non-
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