Production of Isopropanol by improved recombinant strains
The present invention relates to an expression vector including: nucleic acids encoding the polypeptides forming a polypeptide complex having an enzymatic activity suitable for converting acetoacetyl-CoA into acetoacetate; optionally at least one nucleic acid encoding a polypeptide having an enzymatic activity suitable for converting acetoacetate into acetone; and at least one nucleic acid encoding a polypeptide having an enzymatic activity suitable for converting acetone into isopropanol, the expression of said nucleic acids being controlled by a single constitutive promoter located upstream from the aforementioned nucleic acids.
Main Authors: | , , , , |
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Format: | Patent biblioteca |
Language: | English |
Subjects: | Life Science, |
Online Access: | https://research.wur.nl/en/publications/production-of-isopropanol-by-improved-recombinant-strains |
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Summary: | The present invention relates to an expression vector including: nucleic acids encoding the polypeptides forming a polypeptide complex having an enzymatic activity suitable for converting acetoacetyl-CoA into acetoacetate; optionally at least one nucleic acid encoding a polypeptide having an enzymatic activity suitable for converting acetoacetate into acetone; and at least one nucleic acid encoding a polypeptide having an enzymatic activity suitable for converting acetone into isopropanol, the expression of said nucleic acids being controlled by a single constitutive promoter located upstream from the aforementioned nucleic acids. |
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