Production of Isopropanol by improved recombinant strains

The present invention relates to an expression vector including: nucleic acids encoding the polypeptides forming a polypeptide complex having an enzymatic activity suitable for converting acetoacetyl-CoA into acetoacetate; optionally at least one nucleic acid encoding a polypeptide having an enzymatic activity suitable for converting acetoacetate into acetone; and at least one nucleic acid encoding a polypeptide having an enzymatic activity suitable for converting acetone into isopropanol, the expression of said nucleic acids being controlled by a single constitutive promoter located upstream from the aforementioned nucleic acids.

Saved in:
Bibliographic Details
Main Authors: Collas, Florent, Marchal, Rémy, Clement, Benjamin, Lopez Contreras, A.M., Claassen, P.A.M.
Format: Patent biblioteca
Language:English
Subjects:Life Science,
Online Access:https://research.wur.nl/en/publications/production-of-isopropanol-by-improved-recombinant-strains
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present invention relates to an expression vector including: nucleic acids encoding the polypeptides forming a polypeptide complex having an enzymatic activity suitable for converting acetoacetyl-CoA into acetoacetate; optionally at least one nucleic acid encoding a polypeptide having an enzymatic activity suitable for converting acetoacetate into acetone; and at least one nucleic acid encoding a polypeptide having an enzymatic activity suitable for converting acetone into isopropanol, the expression of said nucleic acids being controlled by a single constitutive promoter located upstream from the aforementioned nucleic acids.