Extraction of steviol glycosides from fresh Stevia using acidified water; comparison to hot water extraction, including purification
This report describes a practical comparison of an acidified water extraction of freshly harvested Stevia plants (the NewFoss method) to the hot water extraction of dried Stevia plants, the industry standard. Both extracts are subsequently purified using lab-/bench scale standard industrial technology: coagulation,filtration, adsorption, and ion exchange. The reason for this comparison is the low purity of steviol glycosides reached in tests where an acidified water extract was purified using mainly ultrafiltration in 2014 and 2015. Therefore, the purification potential of the acidified water extract while using standard industrial technology needed to be assessed, in turn in order to show the potential for the acidified water extraction as a viable extraction technology for steviol glycosides from Stevia.The combination of coagulation, filtration, adsorption, and ion exchange results in a 80.6 wt% steviolglycosides in the dry matter of the purified acidified water extraction. This is very close to the 85.4 wt%reached when the same purification processes are performed on a hot water extract. Acidified water extractof fresh Stevia can be purified using the same standard industrial technology, to a similarly high level as ahot water extract of dried Stevia. This strengthens the potential for the acidified water extraction of steviolglycosides from fresh Stevia.
| Main Authors: | , |
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| Format: | External research report biblioteca |
| Language: | English |
| Published: |
ACRRES
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| Subjects: | Life Science, |
| Online Access: | https://research.wur.nl/en/publications/extraction-of-steviol-glycosides-from-fresh-stevia-using-acidifie-2 |
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| Summary: | This report describes a practical comparison of an acidified water extraction of freshly harvested Stevia plants (the NewFoss method) to the hot water extraction of dried Stevia plants, the industry standard. Both extracts are subsequently purified using lab-/bench scale standard industrial technology: coagulation,filtration, adsorption, and ion exchange. The reason for this comparison is the low purity of steviol glycosides reached in tests where an acidified water extract was purified using mainly ultrafiltration in 2014 and 2015. Therefore, the purification potential of the acidified water extract while using standard industrial technology needed to be assessed, in turn in order to show the potential for the acidified water extraction as a viable extraction technology for steviol glycosides from Stevia.The combination of coagulation, filtration, adsorption, and ion exchange results in a 80.6 wt% steviolglycosides in the dry matter of the purified acidified water extraction. This is very close to the 85.4 wt%reached when the same purification processes are performed on a hot water extract. Acidified water extractof fresh Stevia can be purified using the same standard industrial technology, to a similarly high level as ahot water extract of dried Stevia. This strengthens the potential for the acidified water extraction of steviolglycosides from fresh Stevia. |
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