Effective Chikungunya Virus-like Particle Vaccine Produced in Insect Cells

The emerging arthritogenic, mosquito-borne chikungunya virus (CHIKV) causes severe disease in humans and represents a serious public health threat in countries where Aedes spp mosquitoes are present. This study describes for the first time the successful production of CHIKV virus-like particles (VLPs) in insect cells using recombinant baculoviruses. This well-established expression system is rapidly scalable to volumes required for epidemic responses and proved well suited for processing of CHIKV glycoproteins and production of enveloped VLPs. Herein we show that a single immunization with 1 µg of non-adjuvanted CHIKV VLPs induced high titer neutralizing antibody responses and provided complete protection against viraemia and joint inflammation upon challenge with the Réunion Island CHIKV strain in an adult wild-type mouse model of CHIKV disease. CHIKV VLPs produced in insect cells using recombinant baculoviruses thus represents as a new, safe, non-replicating and effective vaccine candidate against CHIKV infections.

Bibliographic Details

Main Authors:	Metz, S.W.H., Gardner, J., Geertsema, C., Le, T.T., Goh, L., Vlak, J.M., Suhrbier, A., Pijlman, G.P.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	baculovirus vectors, dna vaccines, envelope proteins, equine encephalitis-virus, expression system, glycosylation, immunogenicity, inactivated vaccine, infection, opportunities,
Online Access:	https://research.wur.nl/en/publications/effective-chikungunya-virus-like-particle-vaccine-produced-in-ins
Tags:	Add Tag No Tags, Be the first to tag this record!